%0 Journal Article
%A Blombach, B.
%A Schreiner, M. E.
%A Moch, M.
%A Oldiges, M.
%A Eikmanns, B. J.
%T Effect of pyruvate dehydrogenase complex deficiency on L-lysine production with Corynebacterium glutamicum
%J Applied Microbiology and Biotechnology
%V 76
%@ 0175-7598
%C Berlin
%I Springer
%M PreJuSER-59689
%P 615 - 623
%D 2007
%Z Record converted from VDB: 12.11.2012
%X Intracellular precursor supply is a critical factor for amino acid productivity of Corynebacterium glutamicum. To test for the effect of improved pyruvate availability on L-lysine production, we deleted the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex (PDHC) in the L-lysine-producer C. glutamicum DM1729 and characterised the resulting strain DM1729-BB1 for growth and L-lysine production. Compared to the host strain, C. glutamicum DM1729-BB1 showed no PDHC activity, was acetate auxotrophic and, after complete consumption of the available carbon sources glucose and acetate, showed a more than 50% lower substrate-specific biomass yield (0.14 vs 0.33 mol C/mol C), an about fourfold higher biomass-specific L-lysine yield (5.27 vs 1.23 mmol/g cell dry weight) and a more than 40% higher substrate-specific L-lysine yield (0.13 vs 0.09 mol C/mol C). Overexpression of the pyruvate carboxylase or diaminopimelate dehydrogenase genes in C. glutamicum DM1729-BB1 resulted in a further increase in the biomass-specific L-lysine yield by 6 and 56%, respectively. In addition to L-lysine, significant amounts of pyruvate, L-alanine and L-valine were produced by C. glutamicum DM1729-BB1 and its derivatives, suggesting a surplus of precursor availability and a further potential to improve L-lysine production by engineering the L-lysine biosynthetic pathway.
%K Base Sequence
%K Biotechnology
%K Corynebacterium glutamicum: genetics
%K Corynebacterium glutamicum: growth & development
%K Corynebacterium glutamicum: metabolism
%K DNA, Bacterial: genetics
%K Fermentation
%K Gene Deletion
%K Gene Expression
%K Genes, Bacterial
%K Kinetics
%K Lysine: biosynthesis
%K Pyruvate Dehydrogenase Complex: genetics
%K Pyruvate Dehydrogenase Complex: metabolism
%K DNA, Bacterial (NLM Chemicals)
%K Pyruvate Dehydrogenase Complex (NLM Chemicals)
%K Lysine (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:17333167
%U <Go to ISI:>//WOS:000248893700015
%R 10.1007/s00253-007-0904-1
%U https://juser.fz-juelich.de/record/59689