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| Home > Publications database > Effect of pyruvate dehydrogenase complex deficiency on L-lysine production with Corynebacterium glutamicum |
| Home > Publications database > Effect of pyruvate dehydrogenase complex deficiency on L-lysine production with Corynebacterium glutamicum |
| Journal Article | PreJuSER-59689 |
; ; ; ;
2007
Springer
Berlin
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Please use a persistent id in citations: doi:10.1007/s00253-007-0904-1
Abstract: Intracellular precursor supply is a critical factor for amino acid productivity of Corynebacterium glutamicum. To test for the effect of improved pyruvate availability on L-lysine production, we deleted the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex (PDHC) in the L-lysine-producer C. glutamicum DM1729 and characterised the resulting strain DM1729-BB1 for growth and L-lysine production. Compared to the host strain, C. glutamicum DM1729-BB1 showed no PDHC activity, was acetate auxotrophic and, after complete consumption of the available carbon sources glucose and acetate, showed a more than 50% lower substrate-specific biomass yield (0.14 vs 0.33 mol C/mol C), an about fourfold higher biomass-specific L-lysine yield (5.27 vs 1.23 mmol/g cell dry weight) and a more than 40% higher substrate-specific L-lysine yield (0.13 vs 0.09 mol C/mol C). Overexpression of the pyruvate carboxylase or diaminopimelate dehydrogenase genes in C. glutamicum DM1729-BB1 resulted in a further increase in the biomass-specific L-lysine yield by 6 and 56%, respectively. In addition to L-lysine, significant amounts of pyruvate, L-alanine and L-valine were produced by C. glutamicum DM1729-BB1 and its derivatives, suggesting a surplus of precursor availability and a further potential to improve L-lysine production by engineering the L-lysine biosynthetic pathway.
Keyword(s): Base Sequence (MeSH) ; Biotechnology (MeSH) ; Corynebacterium glutamicum: genetics (MeSH) ; Corynebacterium glutamicum: growth & development (MeSH) ; Corynebacterium glutamicum: metabolism (MeSH) ; DNA, Bacterial: genetics (MeSH) ; Fermentation (MeSH) ; Gene Deletion (MeSH) ; Gene Expression (MeSH) ; Genes, Bacterial (MeSH) ; Kinetics (MeSH) ; Lysine: biosynthesis (MeSH) ; Pyruvate Dehydrogenase Complex: genetics (MeSH) ; Pyruvate Dehydrogenase Complex: metabolism (MeSH) ; DNA, Bacterial ; Pyruvate Dehydrogenase Complex ; Lysine ; J ; Corynebacterium glutamicum (auto) ; L-lysine (auto) ; pyruvate dehydrogenase complex (auto) ; L-lysine production (auto)
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