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000059689 0247_ $$2DOI$$a10.1007/s00253-007-0904-1
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000059689 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000059689 1001_ $$0P:(DE-HGF)0$$aBlombach, B.$$b0
000059689 245__ $$aEffect of pyruvate dehydrogenase complex deficiency on L-lysine production with Corynebacterium glutamicum
000059689 260__ $$aBerlin$$bSpringer$$c2007
000059689 300__ $$a615 - 623
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000059689 440_0 $$0555$$aApplied Microbiology and Biotechnology$$v76$$x0175-7598$$y3
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000059689 520__ $$aIntracellular precursor supply is a critical factor for amino acid productivity of Corynebacterium glutamicum. To test for the effect of improved pyruvate availability on L-lysine production, we deleted the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex (PDHC) in the L-lysine-producer C. glutamicum DM1729 and characterised the resulting strain DM1729-BB1 for growth and L-lysine production. Compared to the host strain, C. glutamicum DM1729-BB1 showed no PDHC activity, was acetate auxotrophic and, after complete consumption of the available carbon sources glucose and acetate, showed a more than 50% lower substrate-specific biomass yield (0.14 vs 0.33 mol C/mol C), an about fourfold higher biomass-specific L-lysine yield (5.27 vs 1.23 mmol/g cell dry weight) and a more than 40% higher substrate-specific L-lysine yield (0.13 vs 0.09 mol C/mol C). Overexpression of the pyruvate carboxylase or diaminopimelate dehydrogenase genes in C. glutamicum DM1729-BB1 resulted in a further increase in the biomass-specific L-lysine yield by 6 and 56%, respectively. In addition to L-lysine, significant amounts of pyruvate, L-alanine and L-valine were produced by C. glutamicum DM1729-BB1 and its derivatives, suggesting a surplus of precursor availability and a further potential to improve L-lysine production by engineering the L-lysine biosynthetic pathway.
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000059689 650_2 $$2MeSH$$aBase Sequence
000059689 650_2 $$2MeSH$$aBiotechnology
000059689 650_2 $$2MeSH$$aCorynebacterium glutamicum: genetics
000059689 650_2 $$2MeSH$$aCorynebacterium glutamicum: growth & development
000059689 650_2 $$2MeSH$$aCorynebacterium glutamicum: metabolism
000059689 650_2 $$2MeSH$$aDNA, Bacterial: genetics
000059689 650_2 $$2MeSH$$aFermentation
000059689 650_2 $$2MeSH$$aGene Deletion
000059689 650_2 $$2MeSH$$aGene Expression
000059689 650_2 $$2MeSH$$aGenes, Bacterial
000059689 650_2 $$2MeSH$$aKinetics
000059689 650_2 $$2MeSH$$aLysine: biosynthesis
000059689 650_2 $$2MeSH$$aPyruvate Dehydrogenase Complex: genetics
000059689 650_2 $$2MeSH$$aPyruvate Dehydrogenase Complex: metabolism
000059689 650_7 $$00$$2NLM Chemicals$$aDNA, Bacterial
000059689 650_7 $$00$$2NLM Chemicals$$aPyruvate Dehydrogenase Complex
000059689 650_7 $$056-87-1$$2NLM Chemicals$$aLysine
000059689 650_7 $$2WoSType$$aJ
000059689 65320 $$2Author$$aCorynebacterium glutamicum
000059689 65320 $$2Author$$aL-lysine
000059689 65320 $$2Author$$apyruvate dehydrogenase complex
000059689 65320 $$2Author$$aL-lysine production
000059689 7001_ $$0P:(DE-HGF)0$$aSchreiner, M. E.$$b1
000059689 7001_ $$0P:(DE-Juel1)VDB23977$$aMoch, M.$$b2$$uFZJ
000059689 7001_ $$0P:(DE-Juel1)129053$$aOldiges, M.$$b3$$uFZJ
000059689 7001_ $$0P:(DE-HGF)0$$aEikmanns, B. J.$$b4
000059689 773__ $$0PERI:(DE-600)1464336-4$$a10.1007/s00253-007-0904-1$$gVol. 76, p. 615 - 623$$p615 - 623$$q76<615 - 623$$tApplied Microbiology and Biotechnology$$v76$$x0175-7598$$y2007
000059689 8567_ $$uhttp://dx.doi.org/10.1007/s00253-007-0904-1
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