TY  - JOUR
AU  - Blombach, B.
AU  - Schreiner, M. E.
AU  - Moch, M.
AU  - Oldiges, M.
AU  - Eikmanns, B. J.
TI  - Effect of pyruvate dehydrogenase complex deficiency on L-lysine production with Corynebacterium glutamicum
JO  - Applied Microbiology and Biotechnology
VL  - 76
SN  - 0175-7598
CY  - Berlin
PB  - Springer
M1  - PreJuSER-59689
SP  - 615 - 623
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - Intracellular precursor supply is a critical factor for amino acid productivity of Corynebacterium glutamicum. To test for the effect of improved pyruvate availability on L-lysine production, we deleted the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex (PDHC) in the L-lysine-producer C. glutamicum DM1729 and characterised the resulting strain DM1729-BB1 for growth and L-lysine production. Compared to the host strain, C. glutamicum DM1729-BB1 showed no PDHC activity, was acetate auxotrophic and, after complete consumption of the available carbon sources glucose and acetate, showed a more than 50% lower substrate-specific biomass yield (0.14 vs 0.33 mol C/mol C), an about fourfold higher biomass-specific L-lysine yield (5.27 vs 1.23 mmol/g cell dry weight) and a more than 40% higher substrate-specific L-lysine yield (0.13 vs 0.09 mol C/mol C). Overexpression of the pyruvate carboxylase or diaminopimelate dehydrogenase genes in C. glutamicum DM1729-BB1 resulted in a further increase in the biomass-specific L-lysine yield by 6 and 56%, respectively. In addition to L-lysine, significant amounts of pyruvate, L-alanine and L-valine were produced by C. glutamicum DM1729-BB1 and its derivatives, suggesting a surplus of precursor availability and a further potential to improve L-lysine production by engineering the L-lysine biosynthetic pathway.
KW  - Base Sequence
KW  - Biotechnology
KW  - Corynebacterium glutamicum: genetics
KW  - Corynebacterium glutamicum: growth & development
KW  - Corynebacterium glutamicum: metabolism
KW  - DNA, Bacterial: genetics
KW  - Fermentation
KW  - Gene Deletion
KW  - Gene Expression
KW  - Genes, Bacterial
KW  - Kinetics
KW  - Lysine: biosynthesis
KW  - Pyruvate Dehydrogenase Complex: genetics
KW  - Pyruvate Dehydrogenase Complex: metabolism
KW  - DNA, Bacterial (NLM Chemicals)
KW  - Pyruvate Dehydrogenase Complex (NLM Chemicals)
KW  - Lysine (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17333167
UR  - <Go to ISI:>//WOS:000248893700015
DO  - DOI:10.1007/s00253-007-0904-1
UR  - https://juser.fz-juelich.de/record/59689
ER  -