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000006444 0247_ $$2DOI$$a10.1002/cphc.200900645
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000006444 084__ $$2WoS$$aChemistry, Physical
000006444 084__ $$2WoS$$aPhysics, Atomic, Molecular & Chemical
000006444 1001_ $$0P:(DE-Juel1)VDB75022$$aMonzel, C.$$b0$$uFZJ
000006444 245__ $$aProbing Bio-Membrane Dynamics by Dual-Wavelength Reflection Interference Contrast Microscopy
000006444 260__ $$aWeinheim$$bWiley-VCH Verl.$$c2009
000006444 300__ $$a2828 - 2838
000006444 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000006444 440_0 $$010582$$aChemPhysChem$$v10$$x1439-4235$$y16
000006444 500__ $$aWe thank Dr. Norbert Kirchgessner and Sebastian Houben for helpful discussions on Matlab, Norbert Kirchgessner and Werner Hurttlen for the implementation of the camera calibration setup, and Nico Hampe for the fabrication of microstructured SiO<INF>2</INF> wafers. One of the authors (C. M.) is grateful for the financial support by the Bonn-Cologne Graduate School.
000006444 520__ $$aWe present an improved analysis of reflection interference contrast microscopy (RICM) images, recorded to investigate model membrane systems that mimic cell adhesion. The model systems were giant unilamellar vesicles (GUV) adhering via specific ligand-receptor interactions to supported lipid bilayers (SLB) or to patterns of receptors. Conventional RICM and dual-wavelength RICM (DW-RICM) were applied to measure absolute optical distances between the biomembranes and planar substrates. We developed algorithms for a straightforward implementation of an automated, time-resolved reconstruction of the membrane conformations from RICM/DW-RICM images, taking into account all the interfaces in the system and blurring of the data due to camera noise. Finally, we demonstrate the validity and usefulness of this new approach by analyzing the topography and fluctuations of a bound membrane in the steady state and its dynamic adaptation to osmotic pressure changes. These measurements clearly show that macroscopic membrane flow through tightly adhered area is possible in our system.
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000006444 588__ $$aDataset connected to Web of Science, Pubmed
000006444 650_2 $$2MeSH$$aAlgorithms
000006444 650_2 $$2MeSH$$aMicroscopy, Interference
000006444 650_2 $$2MeSH$$aMolecular Dynamics Simulation
000006444 650_2 $$2MeSH$$aOsmotic Pressure
000006444 650_2 $$2MeSH$$aUnilamellar Liposomes: chemistry
000006444 650_7 $$00$$2NLM Chemicals$$aUnilamellar Liposomes
000006444 650_7 $$2WoSType$$aJ
000006444 65320 $$2Author$$aadhesion
000006444 65320 $$2Author$$ainterfaces
000006444 65320 $$2Author$$anonequilibrium processes
000006444 65320 $$2Author$$areflection interference contrast microscopy
000006444 65320 $$2Author$$avesicles
000006444 7001_ $$0P:(DE-Juel1)VDB68680$$aFenz, S.$$b1$$uFZJ
000006444 7001_ $$0P:(DE-Juel1)128833$$aMerkel, R.$$b2$$uFZJ
000006444 7001_ $$0P:(DE-Juel1)VDB57655$$aSengupta, K.$$b3$$uFZJ
000006444 773__ $$0PERI:(DE-600)2025223-7$$a10.1002/cphc.200900645$$gVol. 10, p. 2828 - 2838$$p2828 - 2838$$q10<2828 - 2838$$tChemPhysChem$$v10$$x1439-4235$$y2009
000006444 8567_ $$uhttp://dx.doi.org/10.1002/cphc.200900645
000006444 909CO $$ooai:juser.fz-juelich.de:6444$$pVDB
000006444 9131_ $$0G:(DE-Juel1)FUEK414$$bMaterie$$kP54$$lKondensierte Materie$$vKondensierte Materie$$x0$$zentfällt   bis 2009
000006444 9141_ $$y2009
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000006444 9201_ $$0I:(DE-Juel1)VDB802$$d31.12.2010$$gIBN$$kIBN-4$$lBiomechanik$$x0
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