Journal Article

PreJuSER-6444

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Probing Bio-Membrane Dynamics by Dual-Wavelength Reflection Interference Contrast Microscopy

Monzel, C.FZJ* ; Fenz, S.FZJ* ; Merkel, R.FZJ* ; Sengupta, K.FZJ*

2009
Wiley-VCH Verl. Weinheim

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Please use a persistent id in citations: doi:10.1002/cphc.200900645

Abstract: We present an improved analysis of reflection interference contrast microscopy (RICM) images, recorded to investigate model membrane systems that mimic cell adhesion. The model systems were giant unilamellar vesicles (GUV) adhering via specific ligand-receptor interactions to supported lipid bilayers (SLB) or to patterns of receptors. Conventional RICM and dual-wavelength RICM (DW-RICM) were applied to measure absolute optical distances between the biomembranes and planar substrates. We developed algorithms for a straightforward implementation of an automated, time-resolved reconstruction of the membrane conformations from RICM/DW-RICM images, taking into account all the interfaces in the system and blurring of the data due to camera noise. Finally, we demonstrate the validity and usefulness of this new approach by analyzing the topography and fluctuations of a bound membrane in the steady state and its dynamic adaptation to osmotic pressure changes. These measurements clearly show that macroscopic membrane flow through tightly adhered area is possible in our system.

Keyword(s): Algorithms (MeSH) ; Microscopy, Interference (MeSH) ; Molecular Dynamics Simulation (MeSH) ; Osmotic Pressure (MeSH) ; Unilamellar Liposomes: chemistry (MeSH) ; Unilamellar Liposomes ; J ; adhesion (auto) ; interfaces (auto) ; nonequilibrium processes (auto) ; reflection interference contrast microscopy (auto) ; vesicles (auto)

Note: We thank Dr. Norbert Kirchgessner and Sebastian Houben for helpful discussions on Matlab, Norbert Kirchgessner and Werner Hurttlen for the implementation of the camera calibration setup, and Nico Hampe for the fabrication of microstructured SiO<INF>2</INF> wafers. One of the authors (C. M.) is grateful for the financial support by the Bonn-Cologne Graduate School.

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Contributing Institute(s):

1. Biomechanik (IBN-4)

Research Program(s):

1. Kondensierte Materie (P54)

Appears in the scientific report 2009

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