TY  - JOUR
AU  - Monzel, C.
AU  - Fenz, S.
AU  - Merkel, R.
AU  - Sengupta, K.
TI  - Probing Bio-Membrane Dynamics by Dual-Wavelength Reflection Interference Contrast Microscopy
JO  - ChemPhysChem
VL  - 10
SN  - 1439-4235
CY  - Weinheim
PB  - Wiley-VCH Verl.
M1  - PreJuSER-6444
SP  - 2828 - 2838
PY  - 2009
N1  - We thank Dr. Norbert Kirchgessner and Sebastian Houben for helpful discussions on Matlab, Norbert Kirchgessner and Werner Hurttlen for the implementation of the camera calibration setup, and Nico Hampe for the fabrication of microstructured SiO<INF>2</INF> wafers. One of the authors (C. M.) is grateful for the financial support by the Bonn-Cologne Graduate School.
AB  - We present an improved analysis of reflection interference contrast microscopy (RICM) images, recorded to investigate model membrane systems that mimic cell adhesion. The model systems were giant unilamellar vesicles (GUV) adhering via specific ligand-receptor interactions to supported lipid bilayers (SLB) or to patterns of receptors. Conventional RICM and dual-wavelength RICM (DW-RICM) were applied to measure absolute optical distances between the biomembranes and planar substrates. We developed algorithms for a straightforward implementation of an automated, time-resolved reconstruction of the membrane conformations from RICM/DW-RICM images, taking into account all the interfaces in the system and blurring of the data due to camera noise. Finally, we demonstrate the validity and usefulness of this new approach by analyzing the topography and fluctuations of a bound membrane in the steady state and its dynamic adaptation to osmotic pressure changes. These measurements clearly show that macroscopic membrane flow through tightly adhered area is possible in our system.
KW  - Algorithms
KW  - Microscopy, Interference
KW  - Molecular Dynamics Simulation
KW  - Osmotic Pressure
KW  - Unilamellar Liposomes: chemistry
KW  - Unilamellar Liposomes (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19821476
UR  - <Go to ISI:>//WOS:000272115200011
DO  - DOI:10.1002/cphc.200900645
UR  - https://juser.fz-juelich.de/record/6444
ER  -