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@ARTICLE{Monzel:6444,
      author       = {Monzel, C. and Fenz, S. and Merkel, R. and Sengupta, K.},
      title        = {{P}robing {B}io-{M}embrane {D}ynamics by
                      {D}ual-{W}avelength {R}eflection {I}nterference {C}ontrast
                      {M}icroscopy},
      journal      = {ChemPhysChem},
      volume       = {10},
      issn         = {1439-4235},
      address      = {Weinheim},
      publisher    = {Wiley-VCH Verl.},
      reportid     = {PreJuSER-6444},
      pages        = {2828 - 2838},
      year         = {2009},
      note         = {We thank Dr. Norbert Kirchgessner and Sebastian Houben for
                      helpful discussions on Matlab, Norbert Kirchgessner and
                      Werner Hurttlen for the implementation of the camera
                      calibration setup, and Nico Hampe for the fabrication of
                      microstructured SiO<INF>2</INF> wafers. One of the authors
                      (C. M.) is grateful for the financial support by the
                      Bonn-Cologne Graduate School.},
      abstract     = {We present an improved analysis of reflection interference
                      contrast microscopy (RICM) images, recorded to investigate
                      model membrane systems that mimic cell adhesion. The model
                      systems were giant unilamellar vesicles (GUV) adhering via
                      specific ligand-receptor interactions to supported lipid
                      bilayers (SLB) or to patterns of receptors. Conventional
                      RICM and dual-wavelength RICM (DW-RICM) were applied to
                      measure absolute optical distances between the biomembranes
                      and planar substrates. We developed algorithms for a
                      straightforward implementation of an automated,
                      time-resolved reconstruction of the membrane conformations
                      from RICM/DW-RICM images, taking into account all the
                      interfaces in the system and blurring of the data due to
                      camera noise. Finally, we demonstrate the validity and
                      usefulness of this new approach by analyzing the topography
                      and fluctuations of a bound membrane in the steady state and
                      its dynamic adaptation to osmotic pressure changes. These
                      measurements clearly show that macroscopic membrane flow
                      through tightly adhered area is possible in our system.},
      keywords     = {Algorithms / Microscopy, Interference / Molecular Dynamics
                      Simulation / Osmotic Pressure / Unilamellar Liposomes:
                      chemistry / Unilamellar Liposomes (NLM Chemicals) / J
                      (WoSType)},
      cin          = {IBN-4},
      ddc          = {540},
      cid          = {I:(DE-Juel1)VDB802},
      pnm          = {Kondensierte Materie},
      pid          = {G:(DE-Juel1)FUEK414},
      shelfmark    = {Chemistry, Physical / Physics, Atomic, Molecular $\&$
                      Chemical},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:19821476},
      UT           = {WOS:000272115200011},
      doi          = {10.1002/cphc.200900645},
      url          = {https://juser.fz-juelich.de/record/6444},
}