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000807644 245__ $$aFast antibody fragment motion: flexible linkers act as entropic spring
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000807644 520__ $$aA flexible linker region between three fragments allows antibodies to adjust their binding sites to an antigen or receptor. Using Neutron Spin Echo Spectroscopy we observed fragment motion on a timescale of 7 ns with motional amplitudes of about 1 nm relative to each other. The mechanistic complexity of the linker region can be described by a spring model with Brownian motion of the fragments in a harmonic potential. Displacements, timescale, friction and force constant of the underlying dynamics are accessed. The force constant exhibits a similar strength to an entropic spring, with friction of the fragment matching the unbound state. The observed fast motions are fluctuations in pre-existing equilibrium configurations. The Brownian motion of domains in a harmonic potential is the appropriate model to examine functional hinge motions dependent on the structural topology and highlights the role of internal forces and friction to function.
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000807644 7001_ $$0P:(DE-Juel1)136923$$aIvanova, Oxana$$b1$$ufzj
000807644 7001_ $$0P:(DE-Juel1)130872$$aOhl, Michael$$b2$$ufzj
000807644 7001_ $$0P:(DE-Juel1)130542$$aBiehl, Ralf$$b3$$eCorresponding author$$ufzj
000807644 7001_ $$0P:(DE-Juel1)130917$$aRichter, Dieter$$b4$$ufzj
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