Journal Article

FZJ-2016-02131

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Fast antibody fragment motion: flexible linkers act as entropic spring

Stingaciu, L.FZJ* ; Ivanova, O.FZJ* ; Ohl, M.FZJ* ; Biehl, R. (Corresponding author)FZJ* ; Richter, D.FZJ*

2016
Nature Publishing Group London

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Please use a persistent id in citations: http://hdl.handle.net/2128/10078  doi:10.1038/srep22148

Abstract: A flexible linker region between three fragments allows antibodies to adjust their binding sites to an antigen or receptor. Using Neutron Spin Echo Spectroscopy we observed fragment motion on a timescale of 7 ns with motional amplitudes of about 1 nm relative to each other. The mechanistic complexity of the linker region can be described by a spring model with Brownian motion of the fragments in a harmonic potential. Displacements, timescale, friction and force constant of the underlying dynamics are accessed. The force constant exhibits a similar strength to an entropic spring, with friction of the fragment matching the unbound state. The observed fast motions are fluctuations in pre-existing equilibrium configurations. The Brownian motion of domains in a harmonic potential is the appropriate model to examine functional hinge motions dependent on the structural topology and highlights the role of internal forces and friction to function.

Keyword(s): Health and Life (1st) ; Health and Life (1st) ; Biology (2nd) ; Soft Condensed Matter (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS (München) ; Jülich Centre for Neutron Science JCNS (München) ; JCNS-FRM-II)
2. Neutronenstreuung (ICS-1)
3. Neutronenstreuung (Neutronenstreuung ; JCNS-1)
4. JCNS-SNS (JCNS-SNS)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)
3. 6G15 - FRM II / MLZ (POF3-6G15) (POF3-6G15)

Experiment(s):

1. J-NSE: Neutron spin-echo spectrometer (NL2ao)

Appears in the scientific report 2016

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Database coverage:
; ; ; ; BIOSIS Previews ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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