Journal Article

FZJ-2016-02239

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Small-Angle Neutron Scattering investigation of cholesterol-doped DMPC liposomes interacting with β-cyclodextrin

Joset, A. (Corresponding author) ; Grammenos, A. ; Hoebeke, M. ; Leyh, B. (Corresponding author)

2016
Springer Science + Business Media B.V Dordrecht [u.a.]

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Please use a persistent id in citations: doi:10.1007/s10847-015-0592-x

Abstract: The Small-Angle Neutron Scattering technique has been applied to characterize the influence of a randomly methylated β-cyclodextrin, called RAMEB, on dimyristoylphosphatidylcholine (DMPC) liposomes doped with cholesterol. From the modelling of the experimental neutron scattering cross sections, the detailed response of the vesicle structure upon addition of increasing amounts of RAMEB up to 30 mM has been assessed. This study has been performed below and above the DMPC bilayer phase transition temperature and shows that cholesterol extraction by RAMEB is linked to a decrease of the average radius and of the aggregation number of the vesicles. This extraction takes place in a dose-dependent way until a more monodisperse population of cholesterol-free liposomes is obtained. In addition, the bilayer thickness evolution was inferred, as well as the liposome coverage by RAMEB.

Keyword(s): Health and Life (1st) ; Health and Life (1st) ; Biology (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS (München) ; Jülich Centre for Neutron Science JCNS (München) ; JCNS-FRM-II)

Research Program(s):

1. 6G15 - FRM II / MLZ (POF3-6G15) (POF3-6G15)
2. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)

Experiment(s):

1. KWS-1: Small angle scattering diffractometer (NL3b)
2. KWS-2: Small angle scattering diffractometer (NL3ao)

Appears in the scientific report 2016

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Database coverage:
; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; No Authors Fulltext ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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