Journal Article

FZJ-2016-04249

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach

Wojtowicz, H. ; Prochnicka-Chalufour, A. ; de Amorim, G. C. ; Roudenko, O. ; Simenel, C. ; Malki, I. ; Pehau-Arnaudet, G. ; Gubellini, F. ; Koutsioumpas, A.FZJ* ; Perez, J. ; Delepelaire, P. ; Delepierre, M. ; Fronzes, R. ; Izadi-Pruneyre, N. (Corresponding author)

2016
Portland Press79505 London

This record in other databases:      

Please use a persistent id in citations: doi:10.1042/BCJ20160131

Abstract: Bacteria use diverse signalling pathways to adapt gene expression to external stimuli. In Gram-negative bacteria, the binding of scarce nutrients to membrane transporters triggers a signalling process that up-regulates the expression of genes of various functions, from uptake of nutrient to production of virulence factors. Although proteins involved in this process have been identified, signal transduction through this family of transporters is not well understood. In the present study, using an integrative approach (EM, SAXS, X-ray crystallography and NMR), we have studied the structure of the haem transporter HasR captured in two stages of the signalling process, i.e. before and after the arrival of signalling activators (haem and its carrier protein). We show for the first time that the HasR domain responsible for signal transfer: (i) is highly flexible in two stages of signalling; (ii) extends into the periplasm at approximately 70–90 Å (1 Å=0.1 nm) from the HasR β-barrel; and (iii) exhibits local conformational changes in response to the arrival of signalling activators. These features would favour the signal transfer from HasR to its cytoplasmic membrane partners.

Keyword(s): Health and Life (1st) ; Health and Life (1st) ; Biology (2nd)

---

Contributing Institute(s):

1. JCNS-FRM-II (JCNS (München) ; Jülich Centre for Neutron Science JCNS (München) ; JCNS-FRM-II)
2. Neutronenstreuung (Neutronenstreuung ; JCNS-1)

Research Program(s):

1. 6G15 - FRM II / MLZ (POF3-6G15) (POF3-6G15)
2. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2016

---

Database coverage:
; BIOSIS Previews ; Current Contents - Life Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

---