TY  - JOUR
AU  - Zhu, Jinsheng
AU  - Bailly, Aur�lien
AU  - Zwiewka, Marta
AU  - Sovero, Valpuri
AU  - di Donato, Martin
AU  - Ge, Pei
AU  - Oehri, Jacqueline
AU  - Aryal, Bibek
AU  - Hao, Pengchao
AU  - Linnert, Miriam
AU  - Burgardt, Noelia
AU  - Lücke, Christian
AU  - Weiwad, Matthias
AU  - Michel, Max
AU  - Weiergräber, Oliver H.
AU  - Pollmann, Stephan
AU  - Azzarello, Elisa
AU  - Mancuso, Stefano
AU  - Ferro, Noel
AU  - Fukao, Yoichiro
AU  - Hoffmann, Celine
AU  - Wedlich-Söldner, Roland
AU  - Friml, Jiri
AU  - Thomas, Clement
AU  - Geisler, Markus
TI  - TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics
JO  - The plant cell
VL  - 28
IS  - 4
SN  - 1040-4651
CY  - Rockville, Md.
PB  - Soc.
M1  - FZJ-2016-04749
SP  - 930-948
PY  - 2016
AB  - Plant growth and architecture is regulated by the polar distribution of the hormone auxin. Polarity and flexibility of this process is provided by constant cycling of auxin transporter vesicles along actin filaments, coordinated by a positive auxin-actin feedback loop. Both polar auxin transport and vesicle cycling are inhibited by synthetic auxin transport inhibitors, such as 1-N-naphthylphthalamic acid (NPA), counteracting the effect of auxin; however, underlying targets and mechanisms are unclear. Using NMR, we map the NPA binding surface on the Arabidopsis ABCB chaperone, TWISTED DWARF1 (TWD1). We identify ACTIN7 as a relevant - although likely indirect - TWD1 interactor, show TWD1-dependent regulation of actin filament organization and dynamics, and that TWD1 is required for NPA-mediated actin cytoskeleton remodeling. The TWD1-ACTIN7 axis controls plasma membrane presence of efflux transporters and as a consequence act7 and twd1 share developmental and physiological phenotypes indicative of defects in auxin transport. These can be phenocopied by NPA treatment or by chemical actin (de)stabilization. We provide evidence that TWD1 determines downstream locations of auxin efflux transporters by adjusting AF de-bundling and dynamizing processes and mediating NPA action on the latter. This function appears to be evolutionary conserved since TWD1 expression in budding yeast alters actin polarization and cell polarity and provides NPA sensitivity.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000375416000012
C6  - pmid:27053424
DO  - DOI:10.1105/tpc.15.00726
UR  - https://juser.fz-juelich.de/record/818275
ER  -