Journal Article

FZJ-2016-04749

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png TWISTED DWARF1 mediates the action of auxin transport inhibitors on actin cytoskeleton dynamics

Zhu, J. ; Bailly, A. ; Zwiewka, M. ; Sovero, V. ; di Donato, M. ; Ge, P. ; Oehri, J. ; Aryal, B. ; Hao, P. ; Linnert, M. ; Burgardt, N. ; Lücke, C. ; Weiwad, M. ; Michel, M. ; Weiergräber, O. H.FZJ* ; Pollmann, S. ; Azzarello, E. ; Mancuso, S. ; Ferro, N. ; Fukao, Y. ; Hoffmann, C. ; Wedlich-Söldner, R. ; Friml, J. ; Thomas, C. ; Geisler, M. (Corresponding author)

2016
Soc. Rockville, Md.

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Please use a persistent id in citations: http://hdl.handle.net/2128/12311  doi:10.1105/tpc.15.00726

Abstract: Plant growth and architecture is regulated by the polar distribution of the hormone auxin. Polarity and flexibility of this process is provided by constant cycling of auxin transporter vesicles along actin filaments, coordinated by a positive auxin-actin feedback loop. Both polar auxin transport and vesicle cycling are inhibited by synthetic auxin transport inhibitors, such as 1-N-naphthylphthalamic acid (NPA), counteracting the effect of auxin; however, underlying targets and mechanisms are unclear. Using NMR, we map the NPA binding surface on the Arabidopsis ABCB chaperone, TWISTED DWARF1 (TWD1). We identify ACTIN7 as a relevant - although likely indirect - TWD1 interactor, show TWD1-dependent regulation of actin filament organization and dynamics, and that TWD1 is required for NPA-mediated actin cytoskeleton remodeling. The TWD1-ACTIN7 axis controls plasma membrane presence of efflux transporters and as a consequence act7 and twd1 share developmental and physiological phenotypes indicative of defects in auxin transport. These can be phenocopied by NPA treatment or by chemical actin (de)stabilization. We provide evidence that TWD1 determines downstream locations of auxin efflux transporters by adjusting AF de-bundling and dynamizing processes and mediating NPA action on the latter. This function appears to be evolutionary conserved since TWD1 expression in budding yeast alters actin polarization and cell polarity and provides NPA sensitivity.

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Contributing Institute(s):

1. Strukturbiochemie (ICS-6)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2016

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Database coverage:
; ; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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