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@ARTICLE{Zhu:818275,
author = {Zhu, Jinsheng and Bailly, Aur�lien and Zwiewka, Marta and
Sovero, Valpuri and di Donato, Martin and Ge, Pei and Oehri,
Jacqueline and Aryal, Bibek and Hao, Pengchao and Linnert,
Miriam and Burgardt, Noelia and Lücke, Christian and
Weiwad, Matthias and Michel, Max and Weiergräber, Oliver H.
and Pollmann, Stephan and Azzarello, Elisa and Mancuso,
Stefano and Ferro, Noel and Fukao, Yoichiro and Hoffmann,
Celine and Wedlich-Söldner, Roland and Friml, Jiri and
Thomas, Clement and Geisler, Markus},
title = {{TWISTED} {DWARF}1 mediates the action of auxin transport
inhibitors on actin cytoskeleton dynamics},
journal = {The plant cell},
volume = {28},
number = {4},
issn = {1040-4651},
address = {Rockville, Md.},
publisher = {Soc.},
reportid = {FZJ-2016-04749},
pages = {930-948},
year = {2016},
abstract = {Plant growth and architecture is regulated by the polar
distribution of the hormone auxin. Polarity and flexibility
of this process is provided by constant cycling of auxin
transporter vesicles along actin filaments, coordinated by a
positive auxin-actin feedback loop. Both polar auxin
transport and vesicle cycling are inhibited by synthetic
auxin transport inhibitors, such as 1-N-naphthylphthalamic
acid (NPA), counteracting the effect of auxin; however,
underlying targets and mechanisms are unclear. Using NMR, we
map the NPA binding surface on the Arabidopsis ABCB
chaperone, TWISTED DWARF1 (TWD1). We identify ACTIN7 as a
relevant - although likely indirect - TWD1 interactor, show
TWD1-dependent regulation of actin filament organization and
dynamics, and that TWD1 is required for NPA-mediated actin
cytoskeleton remodeling. The TWD1-ACTIN7 axis controls
plasma membrane presence of efflux transporters and as a
consequence act7 and twd1 share developmental and
physiological phenotypes indicative of defects in auxin
transport. These can be phenocopied by NPA treatment or by
chemical actin (de)stabilization. We provide evidence that
TWD1 determines downstream locations of auxin efflux
transporters by adjusting AF de-bundling and dynamizing
processes and mediating NPA action on the latter. This
function appears to be evolutionary conserved since TWD1
expression in budding yeast alters actin polarization and
cell polarity and provides NPA sensitivity.},
cin = {ICS-6},
ddc = {570},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-551},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000375416000012},
pubmed = {pmid:27053424},
doi = {10.1105/tpc.15.00726},
url = {https://juser.fz-juelich.de/record/818275},
}