%0 Journal Article
%A Viennet, Thibault
%A Viegas, Aldino
%A Kuepper, Arne
%A Arens, Sabine
%A Gelev, Vladimir
%A Petrov, Ognyan
%A Grossmann, Tom N.
%A Heise, Henrike
%A Etzkorn, Manuel
%T Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization
%J Angewandte Chemie / International edition
%V 55
%N 36
%@ 1433-7851
%C Weinheim
%I Wiley-VCH
%M FZJ-2016-05503
%P 10746 - 10750
%D 2016
%X Nuclear magnetic resonance (NMR) spectroscopy has the intrinsic capabilities to investigate proteins in native environments. In general, however, NMR relies on non-natural protein purity and concentration to increase the desired signal over the background. We here report on the efficient and specific hyperpolarization of low amounts of a target protein in a large isotope-labeled background by combining dynamic nuclear polarization (DNP) and the selectivity of protein interactions. Using a biradical-labeled ligand, we were able to direct the hyperpolarization to the protein of interest, maintaining comparable signal enhancement with about 400-fold less radicals than conventionally used. We could selectively filter out our target protein directly from crude cell lysate obtained from only 8 mL of fully isotope-enriched cell culture. Our approach offers effective means to study proteins with atomic resolution in increasingly native concentrations and environments.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000383473600039
%$ pmid:27351143
%R 10.1002/anie.201603205
%U https://juser.fz-juelich.de/record/819929