Journal Article

FZJ-2016-05503

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization

Viennet, T.FZJ* ; Viegas, A.FZJ* ; Kuepper, A. ; Arens, S. ; Gelev, V. ; Petrov, O. ; Grossmann, T. N. ; Heise, H.FZJ* ; Etzkorn, M. (Corresponding author)

2016
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/anie.201603205

Abstract: Nuclear magnetic resonance (NMR) spectroscopy has the intrinsic capabilities to investigate proteins in native environments. In general, however, NMR relies on non-natural protein purity and concentration to increase the desired signal over the background. We here report on the efficient and specific hyperpolarization of low amounts of a target protein in a large isotope-labeled background by combining dynamic nuclear polarization (DNP) and the selectivity of protein interactions. Using a biradical-labeled ligand, we were able to direct the hyperpolarization to the protein of interest, maintaining comparable signal enhancement with about 400-fold less radicals than conventionally used. We could selectively filter out our target protein directly from crude cell lysate obtained from only 8 mL of fully isotope-enriched cell culture. Our approach offers effective means to study proteins with atomic resolution in increasingly native concentrations and environments.

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Contributing Institute(s):

1. Strukturbiochemie (ICS-6)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2016

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Database coverage:
; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; IF >= 10 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; No Authors Fulltext ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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