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000819929 1001_ $$0P:(DE-Juel1)161253$$aViennet, Thibault$$b0
000819929 245__ $$aSelective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization
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000819929 520__ $$aNuclear magnetic resonance (NMR) spectroscopy has the intrinsic capabilities to investigate proteins in native environments. In general, however, NMR relies on non-natural protein purity and concentration to increase the desired signal over the background. We here report on the efficient and specific hyperpolarization of low amounts of a target protein in a large isotope-labeled background by combining dynamic nuclear polarization (DNP) and the selectivity of protein interactions. Using a biradical-labeled ligand, we were able to direct the hyperpolarization to the protein of interest, maintaining comparable signal enhancement with about 400-fold less radicals than conventionally used. We could selectively filter out our target protein directly from crude cell lysate obtained from only 8 mL of fully isotope-enriched cell culture. Our approach offers effective means to study proteins with atomic resolution in increasingly native concentrations and environments.
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000819929 7001_ $$0P:(DE-Juel1)161140$$aViegas, Aldino$$b1
000819929 7001_ $$0P:(DE-HGF)0$$aKuepper, Arne$$b2
000819929 7001_ $$0P:(DE-HGF)0$$aArens, Sabine$$b3
000819929 7001_ $$0P:(DE-HGF)0$$aGelev, Vladimir$$b4
000819929 7001_ $$0P:(DE-HGF)0$$aPetrov, Ognyan$$b5
000819929 7001_ $$00000-0003-0179-4116$$aGrossmann, Tom N.$$b6
000819929 7001_ $$0P:(DE-Juel1)132002$$aHeise, Henrike$$b7
000819929 7001_ $$00000-0002-9796-3246$$aEtzkorn, Manuel$$b8$$eCorresponding author
000819929 773__ $$0PERI:(DE-600)2011836-3$$a10.1002/anie.201603205$$gVol. 55, no. 36, p. 10746 - 10750$$n36$$p10746 - 10750$$tAngewandte Chemie / International edition$$v55$$x1433-7851$$y2016
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