TY  - JOUR
AU  - Viennet, Thibault
AU  - Viegas, Aldino
AU  - Kuepper, Arne
AU  - Arens, Sabine
AU  - Gelev, Vladimir
AU  - Petrov, Ognyan
AU  - Grossmann, Tom N.
AU  - Heise, Henrike
AU  - Etzkorn, Manuel
TI  - Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization
JO  - Angewandte Chemie / International edition
VL  - 55
IS  - 36
SN  - 1433-7851
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2016-05503
SP  - 10746 - 10750
PY  - 2016
AB  - Nuclear magnetic resonance (NMR) spectroscopy has the intrinsic capabilities to investigate proteins in native environments. In general, however, NMR relies on non-natural protein purity and concentration to increase the desired signal over the background. We here report on the efficient and specific hyperpolarization of low amounts of a target protein in a large isotope-labeled background by combining dynamic nuclear polarization (DNP) and the selectivity of protein interactions. Using a biradical-labeled ligand, we were able to direct the hyperpolarization to the protein of interest, maintaining comparable signal enhancement with about 400-fold less radicals than conventionally used. We could selectively filter out our target protein directly from crude cell lysate obtained from only 8 mL of fully isotope-enriched cell culture. Our approach offers effective means to study proteins with atomic resolution in increasingly native concentrations and environments.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000383473600039
C6  - pmid:27351143
DO  - DOI:10.1002/anie.201603205
UR  - https://juser.fz-juelich.de/record/819929
ER  -