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@ARTICLE{Viennet:819929,
      author       = {Viennet, Thibault and Viegas, Aldino and Kuepper, Arne and
                      Arens, Sabine and Gelev, Vladimir and Petrov, Ognyan and
                      Grossmann, Tom N. and Heise, Henrike and Etzkorn, Manuel},
      title        = {{S}elective {P}rotein {H}yperpolarization in {C}ell
                      {L}ysates {U}sing {T}argeted {D}ynamic {N}uclear
                      {P}olarization},
      journal      = {Angewandte Chemie / International edition},
      volume       = {55},
      number       = {36},
      issn         = {1433-7851},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2016-05503},
      pages        = {10746 - 10750},
      year         = {2016},
      abstract     = {Nuclear magnetic resonance (NMR) spectroscopy has the
                      intrinsic capabilities to investigate proteins in native
                      environments. In general, however, NMR relies on non-natural
                      protein purity and concentration to increase the desired
                      signal over the background. We here report on the efficient
                      and specific hyperpolarization of low amounts of a target
                      protein in a large isotope-labeled background by combining
                      dynamic nuclear polarization (DNP) and the selectivity of
                      protein interactions. Using a biradical-labeled ligand, we
                      were able to direct the hyperpolarization to the protein of
                      interest, maintaining comparable signal enhancement with
                      about 400-fold less radicals than conventionally used. We
                      could selectively filter out our target protein directly
                      from crude cell lysate obtained from only 8 mL of fully
                      isotope-enriched cell culture. Our approach offers effective
                      means to study proteins with atomic resolution in
                      increasingly native concentrations and environments.},
      cin          = {ICS-6},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000383473600039},
      pubmed       = {pmid:27351143},
      doi          = {10.1002/anie.201603205},
      url          = {https://juser.fz-juelich.de/record/819929},
}