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| 001 | 819929 | ||
| 005 | 20210129224419.0 | ||
| 024 | 7 | _ | |a 10.1002/anie.201603205 |2 doi |
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| 100 | 1 | _ | |a Viennet, Thibault |0 P:(DE-Juel1)161253 |b 0 |
| 245 | _ | _ | |a Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization |
| 260 | _ | _ | |a Weinheim |c 2016 |b Wiley-VCH |
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| 520 | _ | _ | |a Nuclear magnetic resonance (NMR) spectroscopy has the intrinsic capabilities to investigate proteins in native environments. In general, however, NMR relies on non-natural protein purity and concentration to increase the desired signal over the background. We here report on the efficient and specific hyperpolarization of low amounts of a target protein in a large isotope-labeled background by combining dynamic nuclear polarization (DNP) and the selectivity of protein interactions. Using a biradical-labeled ligand, we were able to direct the hyperpolarization to the protein of interest, maintaining comparable signal enhancement with about 400-fold less radicals than conventionally used. We could selectively filter out our target protein directly from crude cell lysate obtained from only 8 mL of fully isotope-enriched cell culture. Our approach offers effective means to study proteins with atomic resolution in increasingly native concentrations and environments. |
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| 700 | 1 | _ | |a Arens, Sabine |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Gelev, Vladimir |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Petrov, Ognyan |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Grossmann, Tom N. |0 0000-0003-0179-4116 |b 6 |
| 700 | 1 | _ | |a Heise, Henrike |0 P:(DE-Juel1)132002 |b 7 |
| 700 | 1 | _ | |a Etzkorn, Manuel |0 0000-0002-9796-3246 |b 8 |e Corresponding author |
| 773 | _ | _ | |a 10.1002/anie.201603205 |g Vol. 55, no. 36, p. 10746 - 10750 |0 PERI:(DE-600)2011836-3 |n 36 |p 10746 - 10750 |t Angewandte Chemie / International edition |v 55 |y 2016 |x 1433-7851 |
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