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@ARTICLE{Papagiannopoulos:824287,
author = {Papagiannopoulos, Aristeidis and Meristoudi, Anastasia and
Pispas, Stergios and Radulescu, Aurel},
title = {{M}icelles from {HOOC}-{P}n{BA}- b -{PAA}-{C}$_{12}$
{H}$_{15}$ {D}iblock {A}mphiphilic {P}olyelectrolytes as
{P}rotein {N}anocarriers},
journal = {Biomacromolecules},
volume = {17},
number = {11},
issn = {1526-4602},
address = {Columbus, Ohio},
publisher = {American Chemical Soc.},
reportid = {FZJ-2016-06906},
pages = {3816 - 3827},
year = {2016},
abstract = {We investigate the potential of self-assembled
nanostructures of the PnBA-b-PAA amphiphilic diblock
polyelectrolyte as candidates for protein nanocarriers.
Three PnBA-b-PAA copolymers with different molecular weights
and PnBA/PAA weight ratios are tested. The system with the
most well-defined core–shell micellar structure is chosen
for complexation with lysozyme. Its solutions are found to
contain well-defined core–shell micelles that are stable
upon increase in solution salt content to physiological
levels. Upon mixing with lysozyme we find that the protein
globules accumulate preferably at the outer parts of the
hydrated corona of the micelles. Increasing the protein
concentration, intermicellar aggregation is enhanced in a
controllable way. At high salt content the number of
proteins per micelle is lower compared with the low salt
content, which points to an interaction of predominantly
electrostatic nature. While light scattering is very
sensitive to complexation, small-angle neutron scattering is
able to distinguish between the contributions from
individual micelles and aggregates. This work demonstrates
the use of scattering techniques to characterize
protein–polymer interactions in multiple hierarchical
levels.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / Neutronenstreuung ; JCNS-1 /
ICS-1},
ddc = {540},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106 / I:(DE-Juel1)ICS-1-20110106},
pnm = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)KWS1-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000388155400038},
doi = {10.1021/acs.biomac.6b01408},
url = {https://juser.fz-juelich.de/record/824287},
}