Journal Article

FZJ-2016-06906

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Micelles from HOOC-PnBA- b -PAA-C$_{12}$ H$_{15}$ Diblock Amphiphilic Polyelectrolytes as Protein Nanocarriers

Papagiannopoulos, A. (Corresponding author) ; Meristoudi, A. ; Pispas, S. ; Radulescu, A.FZJ*

2016
American Chemical Soc. Columbus, Ohio

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Please use a persistent id in citations: http://hdl.handle.net/2128/13071  doi:10.1021/acs.biomac.6b01408

Abstract: We investigate the potential of self-assembled nanostructures of the PnBA-b-PAA amphiphilic diblock polyelectrolyte as candidates for protein nanocarriers. Three PnBA-b-PAA copolymers with different molecular weights and PnBA/PAA weight ratios are tested. The system with the most well-defined core–shell micellar structure is chosen for complexation with lysozyme. Its solutions are found to contain well-defined core–shell micelles that are stable upon increase in solution salt content to physiological levels. Upon mixing with lysozyme we find that the protein globules accumulate preferably at the outer parts of the hydrated corona of the micelles. Increasing the protein concentration, intermicellar aggregation is enhanced in a controllable way. At high salt content the number of proteins per micelle is lower compared with the low salt content, which points to an interaction of predominantly electrostatic nature. While light scattering is very sensitive to complexation, small-angle neutron scattering is able to distinguish between the contributions from individual micelles and aggregates. This work demonstrates the use of scattering techniques to characterize protein–polymer interactions in multiple hierarchical levels.

Keyword(s): Polymers, Soft Nano Particles and Proteins (1st) ; Chemistry (2nd) ; Soft Condensed Matter (2nd) ; Biology (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS (München) ; Jülich Centre for Neutron Science JCNS (München) ; JCNS-FRM-II)
2. Neutronenstreuung (Neutronenstreuung ; JCNS-1)
3. Neutronenstreuung (ICS-1)

Research Program(s):

1. 6G15 - FRM II / MLZ (POF3-6G15) (POF3-6G15)
2. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)

Experiment(s):

1. KWS-1: Small angle scattering diffractometer (NL3b)

Appears in the scientific report 2016

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Database coverage:
; ; BIOSIS Previews ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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