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@ARTICLE{Untiet:824942,
author = {Untiet, Verena and Moeller, Lisa M. and Ibarra-Soria,
Ximena and Sánchez-Andrade, Gabriela and Stricker, Miriam
and Neuhaus, Eva M. and Logan, Darren W. and Gensch, Thomas
and Spehr, Marc},
title = {{E}levated {C}ytosolic {C}l − {C}oncentrations in
{D}endritic {K}nobs of {M}ouse {V}omeronasal {S}ensory
{N}eurons},
journal = {Chemical senses},
volume = {41},
number = {8},
issn = {1464-3553},
address = {Oxford},
publisher = {Oxford Univ. Press},
reportid = {FZJ-2016-07438},
pages = {669 - 676},
year = {2016},
abstract = {In rodents, the vomeronasal system controls social and
sexual behavior. However, several mechanistic aspects of
sensory signaling in the vomeronasal organ remain unclear.
Here, we investigate the biophysical basis of a recently
proposed vomeronasal signal transduction component—a Ca 2+
-activated Cl − current. As the physiological role of such
a current is a direct function of the Cl − equilibrium
potential, we determined the intracellular Cl −
concentration in dendritic knobs of vomeronasal neurons.
Quantitative fluorescence lifetime imaging of a Cl −
-sensitive dye at the apical surface of the intact
vomeronasal neuroepithelium revealed increased cytosolic Cl
− levels in dendritic knobs, a substantially lower Cl −
concentration in vomeronasal sustentacular cells, and an
apparent Cl − gradient in vomeronasal neurons along their
dendritic apicobasal axis. Together, our data provide a
biophysical basis for sensory signal amplification in
vomeronasal neuron microvilli by opening Ca 2+ -activated Cl
− channels.},
cin = {ICS-4},
ddc = {540},
cid = {I:(DE-Juel1)ICS-4-20110106},
pnm = {553 - Physical Basis of Diseases (POF3-553)},
pid = {G:(DE-HGF)POF3-553},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000384406500003},
pubmed = {pmid:27377750},
doi = {10.1093/chemse/bjw077},
url = {https://juser.fz-juelich.de/record/824942},
}