Journal Article

FZJ-2016-07438

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Elevated Cytosolic Cl − Concentrations in Dendritic Knobs of Mouse Vomeronasal Sensory Neurons

Untiet, V.FZJ* ; Moeller, L. M. ; Ibarra-Soria, X. ; Sánchez-Andrade, G. ; Stricker, M. ; Neuhaus, E. M. ; Logan, D. W. ; Gensch, T.FZJ* ; Spehr, M. (Corresponding author)

2016
Oxford Univ. Press Oxford

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Please use a persistent id in citations: doi:10.1093/chemse/bjw077

Abstract: In rodents, the vomeronasal system controls social and sexual behavior. However, several mechanistic aspects of sensory signaling in the vomeronasal organ remain unclear. Here, we investigate the biophysical basis of a recently proposed vomeronasal signal transduction component—a Ca 2+ -activated Cl − current. As the physiological role of such a current is a direct function of the Cl − equilibrium potential, we determined the intracellular Cl − concentration in dendritic knobs of vomeronasal neurons. Quantitative fluorescence lifetime imaging of a Cl − -sensitive dye at the apical surface of the intact vomeronasal neuroepithelium revealed increased cytosolic Cl − levels in dendritic knobs, a substantially lower Cl − concentration in vomeronasal sustentacular cells, and an apparent Cl − gradient in vomeronasal neurons along their dendritic apicobasal axis. Together, our data provide a biophysical basis for sensory signal amplification in vomeronasal neuron microvilli by opening Ca 2+ -activated Cl − channels.

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Contributing Institute(s):

1. Zelluläre Biophysik (ICS-4)

Research Program(s):

1. 553 - Physical Basis of Diseases (POF3-553) (POF3-553)

Appears in the scientific report 2016

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Database coverage:
; Allianz-Lizenz / DFG ; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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