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000825205 1001_ $$0P:(DE-HGF)0$$aBotte, Mathieu$$b0
000825205 245__ $$aA central cavity within the holo-translocon suggests a mechanism for membrane protein insertion
000825205 260__ $$aLondon$$bNature Publishing Group$$c2016
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000825205 520__ $$aThe conserved SecYEG protein-conducting channel and the accessory proteins SecDF-YajC and YidC constitute the bacterial holo-translocon (HTL), capable of protein-secretion and membrane-protein insertion. By employing an integrative approach combining small-angle neutron scattering (SANS), low-resolution electron microscopy and biophysical analyses we determined the arrangement of the proteins and lipids within the super-complex. The results guided the placement of X-ray structures of individual HTL components and allowed the proposal of a model of the functional translocon. Their arrangement around a central lipid-containing pool conveys an unexpected, but compelling mechanism for membrane-protein insertion. The periplasmic domains of YidC and SecD are poised at the protein-channel exit-site of SecY, presumably to aid the emergence of translocating polypeptides. The SecY lateral gate for membrane-insertion is adjacent to the membrane ‘insertase’ YidC. Absolute-scale SANS employing a novel contrast-match-point analysis revealed a dynamic complex adopting open and compact configurations around an adaptable central lipid-filled chamber, wherein polytopic membrane-proteins could fold, sheltered from aggregation and proteolysis.
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000825205 7001_ $$0P:(DE-HGF)0$$aZaccai, Nathan R.$$b1
000825205 7001_ $$0P:(DE-HGF)0$$aNijeholt, Jelger Lycklama à.$$b2
000825205 7001_ $$0P:(DE-HGF)0$$aMartin, Remy$$b3
000825205 7001_ $$0P:(DE-HGF)0$$aKnoops, Kèvin$$b4
000825205 7001_ $$0P:(DE-HGF)0$$aPapai, Gabor$$b5
000825205 7001_ $$0P:(DE-HGF)0$$aZou, Juan$$b6
000825205 7001_ $$0P:(DE-HGF)0$$aDeniaud, Aurélien$$b7
000825205 7001_ $$0P:(DE-HGF)0$$aKaruppasamy, Manikandan$$b8
000825205 7001_ $$0P:(DE-HGF)0$$aJiang, Qiyang$$b9
000825205 7001_ $$0P:(DE-HGF)0$$aRoy, Abhishek Singha$$b10
000825205 7001_ $$0P:(DE-HGF)0$$aSchulten, Klaus$$b11
000825205 7001_ $$0P:(DE-HGF)0$$aSchultz, Patrick$$b12
000825205 7001_ $$0P:(DE-HGF)0$$aRappsilber, Juri$$b13
000825205 7001_ $$0P:(DE-HGF)0$$aZaccai, Giuseppe$$b14
000825205 7001_ $$0P:(DE-HGF)0$$aBerger, Imre$$b15
000825205 7001_ $$0P:(DE-HGF)0$$aCollinson, Ian$$b16$$eCorresponding author
000825205 7001_ $$0P:(DE-HGF)0$$aSchaffitzel, Christiane$$b17$$eCorresponding author
000825205 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/srep38399$$gVol. 6, p. 38399 -$$p38399 -$$tScientific reports$$v6$$x2045-2322$$y2016
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