TY  - JOUR
AU  - Botte, Mathieu
AU  - Zaccai, Nathan R.
AU  - Nijeholt, Jelger Lycklama à.
AU  - Martin, Remy
AU  - Knoops, Kèvin
AU  - Papai, Gabor
AU  - Zou, Juan
AU  - Deniaud, Aurélien
AU  - Karuppasamy, Manikandan
AU  - Jiang, Qiyang
AU  - Roy, Abhishek Singha
AU  - Schulten, Klaus
AU  - Schultz, Patrick
AU  - Rappsilber, Juri
AU  - Zaccai, Giuseppe
AU  - Berger, Imre
AU  - Collinson, Ian
AU  - Schaffitzel, Christiane
TI  - A central cavity within the holo-translocon suggests a mechanism for membrane protein insertion
JO  - Scientific reports
VL  - 6
SN  - 2045-2322
CY  - London
PB  - Nature Publishing Group
M1  - FZJ-2016-07676
SP  - 38399 -
PY  - 2016
AB  - The conserved SecYEG protein-conducting channel and the accessory proteins SecDF-YajC and YidC constitute the bacterial holo-translocon (HTL), capable of protein-secretion and membrane-protein insertion. By employing an integrative approach combining small-angle neutron scattering (SANS), low-resolution electron microscopy and biophysical analyses we determined the arrangement of the proteins and lipids within the super-complex. The results guided the placement of X-ray structures of individual HTL components and allowed the proposal of a model of the functional translocon. Their arrangement around a central lipid-containing pool conveys an unexpected, but compelling mechanism for membrane-protein insertion. The periplasmic domains of YidC and SecD are poised at the protein-channel exit-site of SecY, presumably to aid the emergence of translocating polypeptides. The SecY lateral gate for membrane-insertion is adjacent to the membrane ‘insertase’ YidC. Absolute-scale SANS employing a novel contrast-match-point analysis revealed a dynamic complex adopting open and compact configurations around an adaptable central lipid-filled chamber, wherein polytopic membrane-proteins could fold, sheltered from aggregation and proteolysis.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000389740800001
C6  - pmid:27924919
DO  - DOI:10.1038/srep38399
UR  - https://juser.fz-juelich.de/record/825205
ER  -