TY  - JOUR
AU  - Foglia, Fabrizia
AU  - Hazael, Rachael
AU  - Simeoni, Giovanna G.
AU  - Appavou, Marie-Sousai
AU  - Moulin, Martine
AU  - Haertlein, Michael
AU  - Trevor Forsyth, V.
AU  - Seydel, Tilo
AU  - Daniel, Isabelle
AU  - Meersman, Filip
AU  - McMillan, Paul F.
TI  - Water Dynamics in Shewanella oneidensis at Ambient and High Pressure using Quasi-Elastic Neutron Scattering
JO  - Scientific reports
VL  - 6
SN  - 2045-2322
CY  - London
PB  - Nature Publishing Group
M1  - FZJ-2017-01681
SP  - 18862 -
PY  - 2016
AB  - Quasielastic neutron scattering (QENS) is an ideal technique for studying water transport and relaxation dynamics at pico- to nanosecond timescales and at length scales relevant to cellular dimensions. Studies of high pressure dynamic effects in live organisms are needed to understand Earth’s deep biosphere and biotechnology applications. Here we applied QENS to study water transport in Shewanella oneidensis at ambient (0.1 MPa) and high (200 MPa) pressure using H/D isotopic contrast experiments for normal and perdeuterated bacteria and buffer solutions to distinguish intracellular and transmembrane processes. The results indicate that intracellular water dynamics are comparable with bulk diffusion rates in aqueous fluids at ambient conditions but a significant reduction occurs in high pressure mobility. We interpret this as due to enhanced interactions with macromolecules in the nanoconfined environment. Overall diffusion rates across the cell envelope also occur at similar rates but unexpected narrowing of the QENS signal appears between momentum transfer values Q = 0.7–1.1 Å−1 corresponding to real space dimensions of 6–9 Å. The relaxation time increase can be explained by correlated dynamics of molecules passing through Aquaporin water transport complexes located within the inner or outer membrane structures.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000368191200001
C6  - pmid:26738409
DO  - DOI:10.1038/srep18862
UR  - https://juser.fz-juelich.de/record/827561
ER  -