Journal Article

FZJ-2017-01681

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Water Dynamics in Shewanella oneidensis at Ambient and High Pressure using Quasi-Elastic Neutron Scattering

Foglia, F. ; Hazael, R. ; Simeoni, G. G. ; Appavou, M.-S.FZJ* ; Moulin, M. ; Haertlein, M. ; Trevor Forsyth, V. ; Seydel, T. ; Daniel, I. ; Meersman, F. (Corresponding author) ; McMillan, P. F. (Corresponding author)

2016
Nature Publishing Group London

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Please use a persistent id in citations: http://hdl.handle.net/2128/13845  doi:10.1038/srep18862

Abstract: Quasielastic neutron scattering (QENS) is an ideal technique for studying water transport and relaxation dynamics at pico- to nanosecond timescales and at length scales relevant to cellular dimensions. Studies of high pressure dynamic effects in live organisms are needed to understand Earth’s deep biosphere and biotechnology applications. Here we applied QENS to study water transport in Shewanella oneidensis at ambient (0.1 MPa) and high (200 MPa) pressure using H/D isotopic contrast experiments for normal and perdeuterated bacteria and buffer solutions to distinguish intracellular and transmembrane processes. The results indicate that intracellular water dynamics are comparable with bulk diffusion rates in aqueous fluids at ambient conditions but a significant reduction occurs in high pressure mobility. We interpret this as due to enhanced interactions with macromolecules in the nanoconfined environment. Overall diffusion rates across the cell envelope also occur at similar rates but unexpected narrowing of the QENS signal appears between momentum transfer values Q = 0.7–1.1 Å−1 corresponding to real space dimensions of 6–9 Å. The relaxation time increase can be explained by correlated dynamics of molecules passing through Aquaporin water transport complexes located within the inner or outer membrane structures.

Keyword(s): Health and Life (1st) ; Biology (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS (München) ; Jülich Centre for Neutron Science JCNS (München) ; JCNS-FRM-II)
2. Neutronenstreuung (Neutronenstreuung ; JCNS-1)

Research Program(s):

1. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)
2. 6G15 - FRM II / MLZ (POF3-6G15) (POF3-6G15)
3. NMI3-II - Neutron Scattering and Muon Spectroscopy Integrated Initiative (283883) (283883)

Experiment(s):

1. TOFTOF: Cold neutron time-of-flight spectrometer (NL2au)

Appears in the scientific report 2016

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