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@ARTICLE{Yu:827731,
      author       = {Yu, Kun and Yang, Ge and Labahn, Jörg},
      title        = {{H}igh-efficient production and biophysical
                      characterisation of nicastrin, and ist interaction with
                      {APPC}100},
      journal      = {Scientific reports},
      volume       = {7},
      issn         = {2045-2322},
      address      = {London},
      publisher    = {Nature Publishing Group},
      reportid     = {FZJ-2017-01837},
      pages        = {44297},
      year         = {2017},
      abstract     = {Nicastrin, the largest member among the four components of
                      the γ-secretase complex, has been identified to be the
                      substrate recognizer for the proteolytic activity of the
                      complex. Here we report that full-length human nicastrin
                      (hNCT) can be obtained by heterologous expression in E.
                      coli. Milligram quantities of the target protein are
                      purified in a two-step purification protocol using affinity
                      chromatography followed by SEC. The FOS-choline 14 purified
                      tetrameric hNCT exhibits a proper folding with $31\%$
                      α-helix and $23\%$ β-sheet content. Thermal stability
                      studies reveal stable secondary and tertiary structure of
                      the detergent purified hNCT. A physical interaction between
                      nicastrin and the γ-secretase substrate APPC100 confirmed
                      the functionality of hNCT as a substrate recognizer.},
      cin          = {ICS-6},
      ddc          = {000},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000395977600001},
      doi          = {10.1038/srep44297},
      url          = {https://juser.fz-juelich.de/record/827731},
}