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| Home > Publications database > Insights into cell wall structure of Sida hermaphrodita and its influence on recalcitrance |
| Home > Publications database > Insights into cell wall structure of Sida hermaphrodita and its influence on recalcitrance |
| Journal Article | FZJ-2017-02662 |
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2017
Elsevier Science
Amsterdam [u.a.]
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Please use a persistent id in citations: doi:10.1016/j.carbpol.2017.03.062
Abstract: The perennial plant Sida hermaphrodita (Sida) is attracting attention as potential energy crop. Here, thefirst detailed view on non-cellulosic Sida cell wall polysaccharide composition, structure and architectureis given. Cell walls were prepared from Sida stems and sequentially extracted with aqueous buffers andalkali. The structures of the quantitatively predominant polysaccharides present in each fraction weredetermined by biochemical characterization, glycome profiling and mass spectrometry. The amounts ofglucose released by Accellerase-1500®treatment of the cell wall and the cell wall residue remainingafter each extraction were used to assess the roles of pectin and hemicellulose in the recalcitrance of Sidabiomass.4-O-Methyl glucuronoxylan with a low proportion of side substitutions was identified as the majornon-cellulosic glycan component of Sida stem cell walls. Pectic polysaccharides and xylans were foundto be associated with lignin, suggesting that these polysaccharides have roles in Sida cell wall recalcitranceto enzymatic hydrolysis.
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