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@ARTICLE{Baumgart:828816,
      author       = {Baumgart, Meike and Huber, Isabel and Abdollahzadeh, Iman
                      and Gensch, Thomas and Frunzke, Julia},
      title        = {{H}eterologous expression of the {H}alothiobacillus
                      neapolitanus carboxysomal gene cluster in {C}orynebacterium
                      glutamicum},
      journal      = {Journal of biotechnology},
      volume       = {258},
      issn         = {0168-1656},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2017-02672},
      pages        = {126-135},
      year         = {2017},
      abstract     = {Compartmentalization represents a ubiquitous principle used
                      by living organisms to optimize metabolic flux and to avoid
                      detrimental interactions within the cytoplasm. Proteinaceous
                      bacterial microcompartments (BMCs) have therefore created
                      strong interest for the encapsulation of heterologous
                      pathways in microbial model organisms. However, attempts
                      were so far mostly restricted to Escherichia coli. Here, we
                      introduced the carboxysomal gene cluster of Halothiobacillus
                      neapolitanus into the biotechnological platform species
                      Corynebacterium gluta-micum. Transmission electron
                      microscopy, fluorescence microscopy and single molecule
                      localization microscopy suggested the formation of BMC-like
                      structures in cells expressing the complete carboxysome
                      operon or only the shell proteins. Purified carboxysomes
                      consisted of the expected protein components as verified by
                      mass spectrometry. Enzymatic assays revealed the functional
                      production of RuBisCO in C. glutamicum both in the presence
                      and absence of carboxysomal shell proteins. Furthermore, we
                      could show that eYFP is targeted to the carboxysomes by
                      fusion to the large RuBisCO subunit. Overall, this study
                      represents the first transfer of an α‐carboxysomal gene
                      cluster into a Gram-positive model species supporting the
                      modularity and orthogonality of these microcompartments, but
                      also identified important challenges which need to be
                      addressed on the way towards biotechnological application.},
      cin          = {ICS-4 / IBG-1 / ICS-6},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-4-20110106 / I:(DE-Juel1)IBG-1-20101118 /
                      I:(DE-Juel1)ICS-6-20110106},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:28359868},
      UT           = {WOS:000412611100016},
      doi          = {10.1016/j.jbiotec.2017.03.019},
      url          = {https://juser.fz-juelich.de/record/828816},
}