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@ARTICLE{Owen:829362,
author = {Owen, Michael and Csizmadia, Imre G. and Viskolcz, Béla
and Strodel, Birgit},
title = {{P}rotein {S}tability and {U}nfolding {F}ollowing {G}lycine
{R}adical {F}ormation},
journal = {Molecules},
volume = {22},
number = {4},
issn = {1420-3049},
address = {Basel},
publisher = {MDPI},
reportid = {FZJ-2017-03077},
pages = {655},
year = {2017},
abstract = {Glycine (Gly) residues are particularly susceptible to
hydrogen abstraction; which results in the formation of the
capto-dative stabilized Cα-centered Gly radical (GLR) on
the protein backbone. We examined the effect of GLR
formation on the structure of the Trp cage; tryptophan
zipper; and the villin headpiece; three fast-folding and
stable miniproteins; using all-atom (OPLS-AA) molecular
dynamics simulations. Radicalization changes the
conformation of the GLR residue and affects both neighboring
residues but did not affect the stability of the Trp zipper.
The stability of helices away from the radical center in
villin were also affected by radicalization; and GLR in
place of Gly15 caused the Trp cage to unfold within 1 µs.
These results provide new evidence on the destabilizing
effects of protein oxidation by reactive oxygen species.},
cin = {ICS-6},
ddc = {540},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {553 - Physical Basis of Diseases (POF3-553)},
pid = {G:(DE-HGF)POF3-553},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000404517800153},
pubmed = {pmid:28422069},
doi = {10.3390/molecules22040655},
url = {https://juser.fz-juelich.de/record/829362},
}