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@ARTICLE{Wolff:829628,
author = {Wolff, Martin and Zhang-Haagen, Bo and Decker, Christina
and Barz, Bogdan and Schneider, Mario and Biehl, Ralf and
Radulescu, Aurel and Strodel, Birgit and Willbold, Dieter
and Nagel-Steger, Luitgard},
title = {{A}β42 pentamers/hexamers are the smallest detectable
oligomers in solution},
journal = {Scientific reports},
volume = {7},
issn = {2045-2322},
address = {London},
publisher = {Nature Publishing Group},
reportid = {FZJ-2017-03297},
pages = {2493},
year = {2017},
abstract = {Amyloid β (Aβ) oligomers may play a decisive role in
Alzheimer’s disease related neurodegeneration, but their
structural properties are poorly understood. In this report,
sedimentation velocity centrifugation, small angle neutron
scattering (SANS) and molecular modelling were used to
identify the small oligomeric species formed by the 42 amino
acid residue long isoform of Aβ (Aβ42) in solution,
characterized by a sedimentation coefficient of 2.56 S,
and a radius of gyration between 2 and 4 nm. The measured
sedimentation coefficient is in close agreement with the
sedimentation coefficient calculated for Aβ42 hexamers
using MD simulations at µM concentration. To the best of
our knowledge this is the first report detailing the Aβ42
oligomeric species by SANS measurements. Our results
demonstrate that the smallest detectable species in solution
are penta- to hexamers. No evidences for the presence of
dimers, trimers or tetramers were found, although the
existence of those Aβ42 oligomers at measurable quantities
had been reported frequently.},
cin = {ICS-6},
ddc = {000},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {553 - Physical Basis of Diseases (POF3-553)},
pid = {G:(DE-HGF)POF3-553},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000402310900006},
pubmed = {pmid:28559586},
doi = {10.1038/s41598-017-02370-3},
url = {https://juser.fz-juelich.de/record/829628},
}