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000830256 0247_ $$2doi$$a10.1021/acs.cgd.6b01631
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000830256 1001_ $$0P:(DE-Juel1)162102$$aNikolaev, Michael$$b0
000830256 245__ $$aIntegral Membrane Proteins Can Be Crystallized Directly from Nanodiscs
000830256 260__ $$aAmsterdam [u.a.]$$bElsevier$$c2017
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000830256 520__ $$aMembrane-like nanodiscs (ND) have become an important tool for the cell-free expression, solubilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct crystallization of MPs embedded in NDs would be of high importance for structural biology. However, despite considerable efforts we have been as yet unable to obtain crystals suitable for X-ray crystallography. In the present work, we show that an ND-trapped MP can be transferred into the cubic phase and crystallized in meso. Bacteriorhodopsin (BR) reconstituted into nanodiscs was mixed with a lipidic mesophase and crystallization was induced by adding a precipitant. The resulting crystals diffract beyond 1.8 Å. The structure of BR was solved at 1.9 Å and found to be indistinguishable from previous structures obtained with the protein solubilized in detergent. We suggest the proposed protocol of in meso crystallization to be generally applicable to ND-trapped MPs.
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000830256 7001_ $$0P:(DE-HGF)0$$aRound, E.$$b1
000830256 7001_ $$0P:(DE-Juel1)165798$$aGushchin, Ivan$$b2
000830256 7001_ $$0P:(DE-HGF)0$$aPolovinkin, V.$$b3
000830256 7001_ $$0P:(DE-Juel1)131949$$aBalandin, Taras$$b4$$ufzj
000830256 7001_ $$0P:(DE-HGF)0$$aKuzmichev$$b5
000830256 7001_ $$0P:(DE-Juel1)143908$$aShevchenko, Vitaly$$b6
000830256 7001_ $$0P:(DE-Juel1)144613$$aBorshchevskiy, Valentin$$b7
000830256 7001_ $$0P:(DE-HGF)0$$aKuklin, A.$$b8
000830256 7001_ $$0P:(DE-HGF)0$$aRound, A.$$b9
000830256 7001_ $$0P:(DE-HGF)0$$aBernhard, F.$$b10
000830256 7001_ $$0P:(DE-Juel1)132029$$aWillbold, Dieter$$b11$$ufzj
000830256 7001_ $$0P:(DE-Juel1)131957$$aBüldt, Georg$$b12
000830256 7001_ $$0P:(DE-Juel1)131964$$aGordeliy, Valentin$$b13$$eCorresponding author$$ufzj
000830256 773__ $$0PERI:(DE-600)1466514-1$$a10.1021/acs.cgd.6b01631$$n3$$p945–948$$tJournal of crystal growth$$v17$$x0022-0248$$y2017
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