Journal Article

FZJ-2017-03829

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Integral Membrane Proteins Can Be Crystallized Directly from Nanodiscs

Nikolaev, M.FZJ* ; Round, E.FZJ* ; Gushchin, I. ; Polovinkin, V.FZJ* ; Balandin, T.FZJ* ; KuzmichevFZJ* ; Shevchenko, V. ; Borshchevskiy, V. ; Kuklin, A. ; Round, A. ; Bernhard, F. ; Willbold, D.FZJ* ; Büldt, G. ; Gordeliy, V. (Corresponding author)FZJ*

2017
Elsevier Amsterdam [u.a.]

This record in other databases:    

Please use a persistent id in citations: http://hdl.handle.net/2128/14552  doi:10.1021/acs.cgd.6b01631

Abstract: Membrane-like nanodiscs (ND) have become an important tool for the cell-free expression, solubilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct crystallization of MPs embedded in NDs would be of high importance for structural biology. However, despite considerable efforts we have been as yet unable to obtain crystals suitable for X-ray crystallography. In the present work, we show that an ND-trapped MP can be transferred into the cubic phase and crystallized in meso. Bacteriorhodopsin (BR) reconstituted into nanodiscs was mixed with a lipidic mesophase and crystallization was induced by adding a precipitant. The resulting crystals diffract beyond 1.8 Å. The structure of BR was solved at 1.9 Å and found to be indistinguishable from previous structures obtained with the protein solubilized in detergent. We suggest the proposed protocol of in meso crystallization to be generally applicable to ND-trapped MPs.

---

Contributing Institute(s):

1. Strukturbiochemie (ICS-6)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2017

---

Database coverage:
; ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Free to read ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

---