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@ARTICLE{Nikolaev:830256,
      author       = {Nikolaev, Michael and Round, E. and Gushchin, Ivan and
                      Polovinkin, V. and Balandin, Taras and Kuzmichev and
                      Shevchenko, Vitaly and Borshchevskiy, Valentin and Kuklin,
                      A. and Round, A. and Bernhard, F. and Willbold, Dieter and
                      Büldt, Georg and Gordeliy, Valentin},
      title        = {{I}ntegral {M}embrane {P}roteins {C}an {B}e {C}rystallized
                      {D}irectly from {N}anodiscs},
      journal      = {Journal of crystal growth},
      volume       = {17},
      number       = {3},
      issn         = {0022-0248},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {FZJ-2017-03829},
      pages        = {945–948},
      year         = {2017},
      abstract     = {Membrane-like nanodiscs (ND) have become an important tool
                      for the cell-free expression, solubilization, folding, and
                      in vitro structural and functional studies of membrane
                      proteins (MPs). Direct crystallization of MPs embedded in
                      NDs would be of high importance for structural biology.
                      However, despite considerable efforts we have been as yet
                      unable to obtain crystals suitable for X-ray
                      crystallography. In the present work, we show that an
                      ND-trapped MP can be transferred into the cubic phase and
                      crystallized in meso. Bacteriorhodopsin (BR) reconstituted
                      into nanodiscs was mixed with a lipidic mesophase and
                      crystallization was induced by adding a precipitant. The
                      resulting crystals diffract beyond 1.8 Å. The structure of
                      BR was solved at 1.9 Å and found to be indistinguishable
                      from previous structures obtained with the protein
                      solubilized in detergent. We suggest the proposed protocol
                      of in meso crystallization to be generally applicable to
                      ND-trapped MPs.},
      cin          = {ICS-6},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000395493900003},
      doi          = {10.1021/acs.cgd.6b01631},
      url          = {https://juser.fz-juelich.de/record/830256},
}