%0 Journal Article
%A Hundshammer, Christian
%A Düwel, Stephan
%A Köcher, Simone Swantje
%A Gersch, Malte
%A Feuerecker, Benedikt
%A Scheurer, Christoph
%A Haase, Axel
%A Glaser, Steffen J.
%A Schwaiger, Markus
%A Schilling, Franz
%T Deuteration of hyperpolarized 13 c-labelled zymonic and enables sensitivity-enhanced dynamic MRI of pH
%J ChemPhysChem
%V 18
%N 18
%@ 1439-4235
%C Weinheim
%I Wiley-VCH Verl.
%M FZJ-2017-05228
%P 2422–2425
%D 2017
%X Aberrant pH is characteristic of many pathologies such as ischemia, inflammation or cancer. Therefore, a non-invasive and spatially resolved pH determination is valuable for disease diagnosis, characterization of response to treatment and the design of pH-sensitive drug-delivery systems. We recently introduced hyperpolarized [1,5-13C2]zymonic acid (ZA) as a novel MRI probe of extracellular pH utilizing dissolution dynamic polarization (DNP) for a more than 10000-fold signal enhancement of the MRI signal. Here we present a strategy to enhance the sensitivity of this approach by deuteration of ZA yielding [1,5-13C2, 3,6,6,6-D4]zymonic acid (ZAd), which prolongs the liquid state spin lattice relaxation time (T1) by up to 39 % in vitro. Measurements with ZA and ZAd on subcutaneous MAT B III adenocarcinoma in rats show that deuteration increases the signal-to-noise ratio (SNR) by up to 46 % in vivo. Furthermore, we demonstrate a proof of concept for real-time imaging of dynamic pH changes in vitro using ZAd, potentially allowing for the characterization of rapid acidification/basification processes in vivo.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:28719100
%U <Go to ISI:>//WOS:000411193200001
%R 10.1002/cphc.201700779
%U https://juser.fz-juelich.de/record/836104