TY - JOUR
AU - Hundshammer, Christian
AU - Düwel, Stephan
AU - Köcher, Simone Swantje
AU - Gersch, Malte
AU - Feuerecker, Benedikt
AU - Scheurer, Christoph
AU - Haase, Axel
AU - Glaser, Steffen J.
AU - Schwaiger, Markus
AU - Schilling, Franz
TI - Deuteration of hyperpolarized 13 c-labelled zymonic and enables sensitivity-enhanced dynamic MRI of pH
JO - ChemPhysChem
VL - 18
IS - 18
SN - 1439-4235
CY - Weinheim
PB - Wiley-VCH Verl.
M1 - FZJ-2017-05228
SP - 2422–2425
PY - 2017
AB - Aberrant pH is characteristic of many pathologies such as ischemia, inflammation or cancer. Therefore, a non-invasive and spatially resolved pH determination is valuable for disease diagnosis, characterization of response to treatment and the design of pH-sensitive drug-delivery systems. We recently introduced hyperpolarized [1,5-13C2]zymonic acid (ZA) as a novel MRI probe of extracellular pH utilizing dissolution dynamic polarization (DNP) for a more than 10000-fold signal enhancement of the MRI signal. Here we present a strategy to enhance the sensitivity of this approach by deuteration of ZA yielding [1,5-13C2, 3,6,6,6-D4]zymonic acid (ZAd), which prolongs the liquid state spin lattice relaxation time (T1) by up to 39 % in vitro. Measurements with ZA and ZAd on subcutaneous MAT B III adenocarcinoma in rats show that deuteration increases the signal-to-noise ratio (SNR) by up to 46 % in vivo. Furthermore, we demonstrate a proof of concept for real-time imaging of dynamic pH changes in vitro using ZAd, potentially allowing for the characterization of rapid acidification/basification processes in vivo.
LB - PUB:(DE-HGF)16
C6 - pmid:28719100
UR - <Go to ISI:>//WOS:000411193200001
DO - DOI:10.1002/cphc.201700779
UR - https://juser.fz-juelich.de/record/836104
ER -