%0 Journal Article
%A Vetsch, M.
%A Janzik, I.
%A Schaller, A.
%T Characterization of prosystemin expressed in the baculovirus/insect cell system reveals biological activity of the systemin precursor
%J Planta
%V 211
%@ 0032-0935
%C Berlin
%I Springer
%M PreJuSER-8363
%P 91 - 97
%D 2000
%Z Record converted from VDB: 12.11.2012
%X Tomato (Lycopersicon esculentum Mill.) prosystemin in fusion with a viral signal peptide was expressed in Sf21 insect cell cultures after infection with recombinant baculoviruses. Prosystemin was purified from culture supernatants and its identity was confirmed by N-terminal sequence and mass-spectral analyses. Recombinant prosystemin was found to be equally active as compared to systemin in inducing the expression of wound-response genes in tomato plants. In cultured cells of L. peruvianum, prosystemin elicited a rapid alkalinization of the growth medium. The timing and dose-dependence of the alkalinization response were found to be identical for prosystemin and systemin, respectively. Prosystemin-triggered defense responses were inhibited by a competitive antagonist of systemin activity, indicating that the systemin sequence within the primary structure of prosystemin determines its activity.
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000088156400011
%U https://juser.fz-juelich.de/record/8363