Journal Article

PreJuSER-8363

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Characterization of prosystemin expressed in the baculovirus/insect cell system reveals biological activity of the systemin precursor

Vetsch, M. ; Janzik, I.FZJ* ; Schaller, A.

2000
Springer Berlin

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Abstract: Tomato (Lycopersicon esculentum Mill.) prosystemin in fusion with a viral signal peptide was expressed in Sf21 insect cell cultures after infection with recombinant baculoviruses. Prosystemin was purified from culture supernatants and its identity was confirmed by N-terminal sequence and mass-spectral analyses. Recombinant prosystemin was found to be equally active as compared to systemin in inducing the expression of wound-response genes in tomato plants. In cultured cells of L. peruvianum, prosystemin elicited a rapid alkalinization of the growth medium. The timing and dose-dependence of the alkalinization response were found to be identical for prosystemin and systemin, respectively. Prosystemin-triggered defense responses were inhibited by a competitive antagonist of systemin activity, indicating that the systemin sequence within the primary structure of prosystemin determines its activity.

Keyword(s): J ; baculovirus/insect cell expression (auto) ; Lycopersicon (wound response) (auto) ; plant defense (auto) ; prosystemin (auto) ; systemin (auto) ; wound response (auto)

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Biologie des Stoffaustauschs (ICG-6)

Research Program(s):

1. Pflanzenphysiologie des Spurengasaustauschs mit der Atmosphäre (36.61.0)

Appears in the scientific report 2000

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