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000008363 084__ $$2WoS$$aPlant Sciences
000008363 1001_ $$0P:(DE-HGF)0$$aVetsch, M.$$b0
000008363 245__ $$aCharacterization of prosystemin expressed in the baculovirus/insect cell system reveals biological activity of the systemin precursor
000008363 260__ $$aBerlin$$bSpringer$$c2000
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000008363 520__ $$aTomato (Lycopersicon esculentum Mill.) prosystemin in fusion with a viral signal peptide was expressed in Sf21 insect cell cultures after infection with recombinant baculoviruses. Prosystemin was purified from culture supernatants and its identity was confirmed by N-terminal sequence and mass-spectral analyses. Recombinant prosystemin was found to be equally active as compared to systemin in inducing the expression of wound-response genes in tomato plants. In cultured cells of L. peruvianum, prosystemin elicited a rapid alkalinization of the growth medium. The timing and dose-dependence of the alkalinization response were found to be identical for prosystemin and systemin, respectively. Prosystemin-triggered defense responses were inhibited by a competitive antagonist of systemin activity, indicating that the systemin sequence within the primary structure of prosystemin determines its activity.
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000008363 65320 $$2Author$$abaculovirus/insect cell expression
000008363 65320 $$2Author$$aLycopersicon (wound response)
000008363 65320 $$2Author$$aplant defense
000008363 65320 $$2Author$$aprosystemin
000008363 65320 $$2Author$$asystemin
000008363 65320 $$2Author$$awound response
000008363 7001_ $$0P:(DE-Juel1)129338$$aJanzik, I.$$b1$$uFZJ
000008363 7001_ $$0P:(DE-HGF)0$$aSchaller, A.$$b2
000008363 773__ $$0PERI:(DE-600)1463030-8$$gVol. 211, p. 91 - 97$$p91 - 97$$q211<91 - 97$$tPlanta$$v211$$x0032-0935$$y2000
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