TY  - JOUR
AU  - Vetsch, M.
AU  - Janzik, I.
AU  - Schaller, A.
TI  - Characterization of prosystemin expressed in the baculovirus/insect cell system reveals biological activity of the systemin precursor
JO  - Planta
VL  - 211
SN  - 0032-0935
CY  - Berlin
PB  - Springer
M1  - PreJuSER-8363
SP  - 91 - 97
PY  - 2000
N1  - Record converted from VDB: 12.11.2012
AB  - Tomato (Lycopersicon esculentum Mill.) prosystemin in fusion with a viral signal peptide was expressed in Sf21 insect cell cultures after infection with recombinant baculoviruses. Prosystemin was purified from culture supernatants and its identity was confirmed by N-terminal sequence and mass-spectral analyses. Recombinant prosystemin was found to be equally active as compared to systemin in inducing the expression of wound-response genes in tomato plants. In cultured cells of L. peruvianum, prosystemin elicited a rapid alkalinization of the growth medium. The timing and dose-dependence of the alkalinization response were found to be identical for prosystemin and systemin, respectively. Prosystemin-triggered defense responses were inhibited by a competitive antagonist of systemin activity, indicating that the systemin sequence within the primary structure of prosystemin determines its activity.
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000088156400011
UR  - https://juser.fz-juelich.de/record/8363
ER  -