Home > Publications database > Characterization of prosystemin expressed in the baculovirus/insect cell system reveals biological activity of the systemin precursor > print |
001 | 8363 | ||
005 | 20170601190739.0 | ||
024 | 7 | _ | |2 WOS |a WOS:000088156400011 |
037 | _ | _ | |a PreJuSER-8363 |
041 | _ | _ | |a eng |
082 | _ | _ | |a 580 |
084 | _ | _ | |2 WoS |a Plant Sciences |
100 | 1 | _ | |a Vetsch, M. |0 P:(DE-HGF)0 |b 0 |
245 | _ | _ | |a Characterization of prosystemin expressed in the baculovirus/insect cell system reveals biological activity of the systemin precursor |
260 | _ | _ | |a Berlin |b Springer |c 2000 |
300 | _ | _ | |a 91 - 97 |
336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
336 | 7 | _ | |a ARTICLE |2 BibTeX |
336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
336 | 7 | _ | |a article |2 DRIVER |
440 | _ | 0 | |a Planta |x 0032-0935 |0 4992 |v 211 |
500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
520 | _ | _ | |a Tomato (Lycopersicon esculentum Mill.) prosystemin in fusion with a viral signal peptide was expressed in Sf21 insect cell cultures after infection with recombinant baculoviruses. Prosystemin was purified from culture supernatants and its identity was confirmed by N-terminal sequence and mass-spectral analyses. Recombinant prosystemin was found to be equally active as compared to systemin in inducing the expression of wound-response genes in tomato plants. In cultured cells of L. peruvianum, prosystemin elicited a rapid alkalinization of the growth medium. The timing and dose-dependence of the alkalinization response were found to be identical for prosystemin and systemin, respectively. Prosystemin-triggered defense responses were inhibited by a competitive antagonist of systemin activity, indicating that the systemin sequence within the primary structure of prosystemin determines its activity. |
536 | _ | _ | |a Pflanzenphysiologie des Spurengasaustauschs mit der Atmosphäre |c 36.61.0 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK82 |x 0 |
588 | _ | _ | |a Dataset connected to Web of Science |
650 | _ | 7 | |a J |2 WoSType |
653 | 2 | 0 | |2 Author |a baculovirus/insect cell expression |
653 | 2 | 0 | |2 Author |a Lycopersicon (wound response) |
653 | 2 | 0 | |2 Author |a plant defense |
653 | 2 | 0 | |2 Author |a prosystemin |
653 | 2 | 0 | |2 Author |a systemin |
653 | 2 | 0 | |2 Author |a wound response |
700 | 1 | _ | |a Janzik, I. |0 P:(DE-Juel1)129338 |b 1 |u FZJ |
700 | 1 | _ | |a Schaller, A. |0 P:(DE-HGF)0 |b 2 |
773 | _ | _ | |g Vol. 211, p. 91 - 97 |p 91 - 97 |q 211<91 - 97 |0 PERI:(DE-600)1463030-8 |t Planta |v 211 |y 2000 |x 0032-0935 |
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913 | 1 | _ | |k 36.61.0 |v Pflanzenphysiologie des Spurengasaustauschs mit der Atmosphäre |l Umweltforschung |b Umweltvorsorgeforschung |0 G:(DE-Juel1)FUEK82 |x 0 |
914 | 1 | _ | |y 2000 |
915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
920 | 1 | _ | |k ICG-6 |l Biologie des Stoffaustauschs |d 31.12.2001 |g ICG-6 |0 I:(DE-Juel1)VDB144 |x 0 |
970 | _ | _ | |a VDB:(DE-Juel1)11728 |
980 | _ | _ | |a VDB |
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980 | _ | _ | |a journal |
980 | _ | _ | |a I:(DE-Juel1)VDB144 |
980 | _ | _ | |a UNRESTRICTED |
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