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037 _ _ |a PreJuSER-8363
041 _ _ |a eng
082 _ _ |a 580
084 _ _ |2 WoS
|a Plant Sciences
100 1 _ |a Vetsch, M.
|0 P:(DE-HGF)0
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245 _ _ |a Characterization of prosystemin expressed in the baculovirus/insect cell system reveals biological activity of the systemin precursor
260 _ _ |a Berlin
|b Springer
|c 2000
300 _ _ |a 91 - 97
336 7 _ |a Journal Article
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336 7 _ |a Journal Article
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336 7 _ |a article
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440 _ 0 |a Planta
|x 0032-0935
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|v 211
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a Tomato (Lycopersicon esculentum Mill.) prosystemin in fusion with a viral signal peptide was expressed in Sf21 insect cell cultures after infection with recombinant baculoviruses. Prosystemin was purified from culture supernatants and its identity was confirmed by N-terminal sequence and mass-spectral analyses. Recombinant prosystemin was found to be equally active as compared to systemin in inducing the expression of wound-response genes in tomato plants. In cultured cells of L. peruvianum, prosystemin elicited a rapid alkalinization of the growth medium. The timing and dose-dependence of the alkalinization response were found to be identical for prosystemin and systemin, respectively. Prosystemin-triggered defense responses were inhibited by a competitive antagonist of systemin activity, indicating that the systemin sequence within the primary structure of prosystemin determines its activity.
536 _ _ |a Pflanzenphysiologie des Spurengasaustauschs mit der Atmosphäre
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588 _ _ |a Dataset connected to Web of Science
650 _ 7 |a J
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653 2 0 |2 Author
|a baculovirus/insect cell expression
653 2 0 |2 Author
|a Lycopersicon (wound response)
653 2 0 |2 Author
|a plant defense
653 2 0 |2 Author
|a prosystemin
653 2 0 |2 Author
|a systemin
653 2 0 |2 Author
|a wound response
700 1 _ |a Janzik, I.
|0 P:(DE-Juel1)129338
|b 1
|u FZJ
700 1 _ |a Schaller, A.
|0 P:(DE-HGF)0
|b 2
773 _ _ |g Vol. 211, p. 91 - 97
|p 91 - 97
|q 211<91 - 97
|0 PERI:(DE-600)1463030-8
|t Planta
|v 211
|y 2000
|x 0032-0935
909 C O |o oai:juser.fz-juelich.de:8363
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913 1 _ |k 36.61.0
|v Pflanzenphysiologie des Spurengasaustauschs mit der Atmosphäre
|l Umweltforschung
|b Umweltvorsorgeforschung
|0 G:(DE-Juel1)FUEK82
|x 0
914 1 _ |y 2000
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |k ICG-6
|l Biologie des Stoffaustauschs
|d 31.12.2001
|g ICG-6
|0 I:(DE-Juel1)VDB144
|x 0
970 _ _ |a VDB:(DE-Juel1)11728
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980 _ _ |a I:(DE-Juel1)VDB144
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