%0 Journal Article
%A Feng, Lingyan
%A Lyu, Zhaozi
%A Offenhäusser, Andreas
%A Mayer, Dirk
%T Electrochemically triggered aptamer immobilization via click reaction for vascular endothelial growth factor detection
%J Engineering in life sciences
%V 16
%N 6
%@ 1618-0240
%C Weinheim
%I Wiley-VCH
%M FZJ-2017-06401
%P 550 - 559
%D 2016
%X The vascular endothelial growth factor 165 (VEGF165) is widely used as an important biomarker in cancer- and neuron-related diseases. Herein, an aptamer-based biosensor is developed that features effective protein detection. A DNA aptamer is immobilized on a gold electrode surface as recognition element utilizing an electrochemically triggered click reaction. By adjusting the applied cathodic potential, the copper catalyst can be in-situ generated and induce a [3 + 2] cycloaddition reaction between the alkyne-modified aptamer and the azide-functionalized electrode surface. Compared to the commonly used thiol-based aptamer immobilization, the present strategy facilitates a high surface probe density to (1.6 ± 0.12) × 1012 molecules/cm2 in a short period (30 min), long-time stability (at least 1 month), as well as regenerative detection performance with an 84% current recovery in each regeneration cycle. Our work reports on a versatile strategy for the fabrication of VEGF165 aptamer-based biosensors, which can be transferred to other aptamer-based sensors and provides an alternative route for the immobilization of aptamer molecules to sensor surfaces.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000386155800007
%R 10.1002/elsc.201600068
%U https://juser.fz-juelich.de/record/837505