Journal Article

FZJ-2017-06401

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Electrochemically triggered aptamer immobilization via click reaction for vascular endothelial growth factor detection

Feng, L.FZJ* ; Lyu, Z.FZJ* ; Offenhäusser, A.FZJ* ; Mayer, D. (Corresponding author)FZJ*

2016
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/elsc.201600068

Abstract: The vascular endothelial growth factor 165 (VEGF165) is widely used as an important biomarker in cancer- and neuron-related diseases. Herein, an aptamer-based biosensor is developed that features effective protein detection. A DNA aptamer is immobilized on a gold electrode surface as recognition element utilizing an electrochemically triggered click reaction. By adjusting the applied cathodic potential, the copper catalyst can be in-situ generated and induce a [3 + 2] cycloaddition reaction between the alkyne-modified aptamer and the azide-functionalized electrode surface. Compared to the commonly used thiol-based aptamer immobilization, the present strategy facilitates a high surface probe density to (1.6 ± 0.12) × 1012 molecules/cm2 in a short period (30 min), long-time stability (at least 1 month), as well as regenerative detection performance with an 84% current recovery in each regeneration cycle. Our work reports on a versatile strategy for the fabrication of VEGF165 aptamer-based biosensors, which can be transferred to other aptamer-based sensors and provides an alternative route for the immobilization of aptamer molecules to sensor surfaces.

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Contributing Institute(s):

1. Bioelektronik (ICS-8)

Research Program(s):

1. 523 - Controlling Configuration-Based Phenomena (POF3-523) (POF3-523)

Appears in the scientific report 2017

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Database coverage:
; BIOSIS Previews ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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