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000837505 0247_ $$2doi$$a10.1002/elsc.201600068
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000837505 1001_ $$0P:(DE-Juel1)157885$$aFeng, Lingyan$$b0
000837505 245__ $$aElectrochemically triggered aptamer immobilization via click reaction for vascular endothelial growth factor detection
000837505 260__ $$aWeinheim$$bWiley-VCH$$c2016
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000837505 520__ $$aThe vascular endothelial growth factor 165 (VEGF165) is widely used as an important biomarker in cancer- and neuron-related diseases. Herein, an aptamer-based biosensor is developed that features effective protein detection. A DNA aptamer is immobilized on a gold electrode surface as recognition element utilizing an electrochemically triggered click reaction. By adjusting the applied cathodic potential, the copper catalyst can be in-situ generated and induce a [3 + 2] cycloaddition reaction between the alkyne-modified aptamer and the azide-functionalized electrode surface. Compared to the commonly used thiol-based aptamer immobilization, the present strategy facilitates a high surface probe density to (1.6 ± 0.12) × 1012 molecules/cm2 in a short period (30 min), long-time stability (at least 1 month), as well as regenerative detection performance with an 84% current recovery in each regeneration cycle. Our work reports on a versatile strategy for the fabrication of VEGF165 aptamer-based biosensors, which can be transferred to other aptamer-based sensors and provides an alternative route for the immobilization of aptamer molecules to sensor surfaces.
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000837505 7001_ $$0P:(DE-Juel1)162320$$aLyu, Zhaozi$$b1
000837505 7001_ $$0P:(DE-Juel1)128713$$aOffenhäusser, Andreas$$b2
000837505 7001_ $$0P:(DE-Juel1)128707$$aMayer, Dirk$$b3$$eCorresponding author
000837505 773__ $$0PERI:(DE-600)2071199-2$$a10.1002/elsc.201600068$$gVol. 16, no. 6, p. 550 - 559$$n6$$p550 - 559$$tEngineering in life sciences$$v16$$x1618-0240$$y2016
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