TY  - JOUR
AU  - Feng, Lingyan
AU  - Lyu, Zhaozi
AU  - Offenhäusser, Andreas
AU  - Mayer, Dirk
TI  - Electrochemically triggered aptamer immobilization via click reaction for vascular endothelial growth factor detection
JO  - Engineering in life sciences
VL  - 16
IS  - 6
SN  - 1618-0240
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2017-06401
SP  - 550 - 559
PY  - 2016
AB  - The vascular endothelial growth factor 165 (VEGF165) is widely used as an important biomarker in cancer- and neuron-related diseases. Herein, an aptamer-based biosensor is developed that features effective protein detection. A DNA aptamer is immobilized on a gold electrode surface as recognition element utilizing an electrochemically triggered click reaction. By adjusting the applied cathodic potential, the copper catalyst can be in-situ generated and induce a [3 + 2] cycloaddition reaction between the alkyne-modified aptamer and the azide-functionalized electrode surface. Compared to the commonly used thiol-based aptamer immobilization, the present strategy facilitates a high surface probe density to (1.6 ± 0.12) × 1012 molecules/cm2 in a short period (30 min), long-time stability (at least 1 month), as well as regenerative detection performance with an 84% current recovery in each regeneration cycle. Our work reports on a versatile strategy for the fabrication of VEGF165 aptamer-based biosensors, which can be transferred to other aptamer-based sensors and provides an alternative route for the immobilization of aptamer molecules to sensor surfaces.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000386155800007
DO  - DOI:10.1002/elsc.201600068
UR  - https://juser.fz-juelich.de/record/837505
ER  -