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@ARTICLE{Feng:837505,
      author       = {Feng, Lingyan and Lyu, Zhaozi and Offenhäusser, Andreas
                      and Mayer, Dirk},
      title        = {{E}lectrochemically triggered aptamer immobilization via
                      click reaction for vascular endothelial growth factor
                      detection},
      journal      = {Engineering in life sciences},
      volume       = {16},
      number       = {6},
      issn         = {1618-0240},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2017-06401},
      pages        = {550 - 559},
      year         = {2016},
      abstract     = {The vascular endothelial growth factor 165 (VEGF165) is
                      widely used as an important biomarker in cancer- and
                      neuron-related diseases. Herein, an aptamer-based biosensor
                      is developed that features effective protein detection. A
                      DNA aptamer is immobilized on a gold electrode surface as
                      recognition element utilizing an electrochemically triggered
                      click reaction. By adjusting the applied cathodic potential,
                      the copper catalyst can be in-situ generated and induce a [3
                      + 2] cycloaddition reaction between the alkyne-modified
                      aptamer and the azide-functionalized electrode surface.
                      Compared to the commonly used thiol-based aptamer
                      immobilization, the present strategy facilitates a high
                      surface probe density to (1.6 ± 0.12) × 1012 molecules/cm2
                      in a short period (30 min), long-time stability (at least 1
                      month), as well as regenerative detection performance with
                      an $84\%$ current recovery in each regeneration cycle. Our
                      work reports on a versatile strategy for the fabrication of
                      VEGF165 aptamer-based biosensors, which can be transferred
                      to other aptamer-based sensors and provides an alternative
                      route for the immobilization of aptamer molecules to sensor
                      surfaces.},
      cin          = {ICS-8},
      ddc          = {660},
      cid          = {I:(DE-Juel1)ICS-8-20110106},
      pnm          = {523 - Controlling Configuration-Based Phenomena (POF3-523)},
      pid          = {G:(DE-HGF)POF3-523},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000386155800007},
      doi          = {10.1002/elsc.201600068},
      url          = {https://juser.fz-juelich.de/record/837505},
}