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| 001 | 837505 | ||
| 005 | 20240619091228.0 | ||
| 024 | 7 | _ | |a 10.1002/elsc.201600068 |2 doi |
| 024 | 7 | _ | |a 1618-0240 |2 ISSN |
| 024 | 7 | _ | |a 1618-2863 |2 ISSN |
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| 037 | _ | _ | |a FZJ-2017-06401 |
| 082 | _ | _ | |a 660 |
| 100 | 1 | _ | |a Feng, Lingyan |0 P:(DE-Juel1)157885 |b 0 |
| 245 | _ | _ | |a Electrochemically triggered aptamer immobilization via click reaction for vascular endothelial growth factor detection |
| 260 | _ | _ | |a Weinheim |c 2016 |b Wiley-VCH |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
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| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a The vascular endothelial growth factor 165 (VEGF165) is widely used as an important biomarker in cancer- and neuron-related diseases. Herein, an aptamer-based biosensor is developed that features effective protein detection. A DNA aptamer is immobilized on a gold electrode surface as recognition element utilizing an electrochemically triggered click reaction. By adjusting the applied cathodic potential, the copper catalyst can be in-situ generated and induce a [3 + 2] cycloaddition reaction between the alkyne-modified aptamer and the azide-functionalized electrode surface. Compared to the commonly used thiol-based aptamer immobilization, the present strategy facilitates a high surface probe density to (1.6 ± 0.12) × 1012 molecules/cm2 in a short period (30 min), long-time stability (at least 1 month), as well as regenerative detection performance with an 84% current recovery in each regeneration cycle. Our work reports on a versatile strategy for the fabrication of VEGF165 aptamer-based biosensors, which can be transferred to other aptamer-based sensors and provides an alternative route for the immobilization of aptamer molecules to sensor surfaces. |
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| 700 | 1 | _ | |a Lyu, Zhaozi |0 P:(DE-Juel1)162320 |b 1 |
| 700 | 1 | _ | |a Offenhäusser, Andreas |0 P:(DE-Juel1)128713 |b 2 |
| 700 | 1 | _ | |a Mayer, Dirk |0 P:(DE-Juel1)128707 |b 3 |e Corresponding author |
| 773 | _ | _ | |a 10.1002/elsc.201600068 |g Vol. 16, no. 6, p. 550 - 559 |0 PERI:(DE-600)2071199-2 |n 6 |p 550 - 559 |t Engineering in life sciences |v 16 |y 2016 |x 1618-0240 |
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